WC1 Antibody | CC101

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WC1 Antibody | CC101 gallery image 1

Bovine peripheral blood lymphocytes stained with Mouse anti Bovine WC1 followed by Goat anti Mouse IgG (H/L):FITC (STAR117F)

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  • Product Type
    Monoclonal Antibody
  • Clone
    CC101
  • Isotype
    IgG2a
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1655GAC, F, IPdatasheet pdfdatasheet pdf0.1 mg
    MCA1655GA
    MCA1655FFdatasheet pdfdatasheet pdf0.1 mg
    MCA1655F
    MCA1655GC, IPdatasheet pdfdatasheet pdf0.25 mg
    MCA1655G
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Bovine WC1 antibody, clone CC101, recognizes a subset of WC1+ T-cells expressing the WC1.1 isoform (MacHugh, N. et al. 1993). The bovine WC1 cell surface antigen is expressed by a population of gamma/delta T-cells that lack CD2, CD4 and CD8, but express CD3. WC1 expression appears to be heterogeneous and antibodies to this cluster show differing reaction patterns (Crocker, G. et al. 1993).

      Mouse anti bovine WC1, clone CC101, immunoprecipitates a 215 kDa molecule from bovine cells and also recognizes the swine homolog of WC1, which is a 180 kDa molecule. In pigs, the 180 kDa molecule is expressed by a gamma/delta TCR positive T-cell population that also lack CD2, CD4 and CD8 (Carr, M.M. et al. 1994).
    • Intended Use
    • Target Species
      Bovine
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Pigyes
      Sheepyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
      Purified IgG - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      Pack Size: 0.25 mg
      0.09%Sodium Azide
      Pack Size: 0.1 mg0.09% Sodium Azide (NaN3)
      Pack Size: 0.25 mg
      0.09%Sodium Azide
      Pack Size: 0.1 mg0.09% Sodium Azide (NaN3)
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
    • Immunogen
      Con A stimulated bovine lymphocytes
    • Purity
    • Approx. Protein Concentrations
      Pack Size: 0.25 mgIgG concentration 1 mg/ml
      Pack Size: 0.1 mgIgG concentration 1.0 mg/ml
      Pack Size: 0.25 mgIgG concentration 1 mg/ml
      Pack Size: 0.1 mgIgG concentration 1.0 mg/ml
      IgG concentration 0.1 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      extracellular region
      membrane
      scavenger receptor activity
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen
      Immunoprecipitation
      Flow Cytometry
    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen
      Immunoprecipitation
      Flow Cytometry
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional WC1 Antibody Formats

    Formats Clone Applications Sizes available
    WC1 Antibody : FITC CC101 F 0.1 mg
    WC1 Antibody : Purified CC101 C, F, IP 0.1 mg | 0.25 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

              Further Reading

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