Ubiquitin Antibody | Ubi-1 (242.9)

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  • Mouse anti Bovine Ubiquitin
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    Ubi-1 (242.9)
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    9400-1004C, P, WBdatasheet pdfdatasheet pdf0.1 ml
    9400-1004
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti bovine ubiquitin antibody, clone Ubi-1 is specific for ubiquitin an 8.5 kDa, 76 amino acid protein, highly conserved in eukaryotes. Clone Ubi-1 shows staining of tangle material in Alzheimer affected brain, and also stains other pathological inclusions such as Lewy bodies (in Parkinson's disease) and Pick bodies (in Pick's disease).
    • Intended Use
    • Target Species
      Bovine
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Humanyes
      Mouseyes
      Ratyes
      ChickenExpected from Sequence
      DrosophilaExpected from Sequence
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Ascites
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
      0.06% Sodium Azide (NaN3)
    • Immunogen
      Purified ubiquitin (Bovine RBC) conjugated to Keyhole Limpet Haemocyanin.
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen1/2501/1000
      Immunohistology - Paraffin1/2501/1000
      Western Blotting1/2501/1000

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
      Alzheimers' diseased brain
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Ubiquitin Antibody Formats

    Formats Clone Applications Sizes available
    Ubiquitin Antibody : Ascites Ubi-1 (242.9) C, P, WB 0.1 ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Human anti Mouse IgG1:HRPHCA036P0.1 mgE
      HCA036P
      Rat anti Mouse IgG Heavy ChainMCA4240.25 mgE
      MCA424
      Rat anti Mouse IgG1MCA12891 mgE
      MCA1289

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Alzheimers' diseased brain

              References

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