Osteocalcin is a non-collagenous protein found in bone, where it comprises up to 2% of the total bone protein. It binds strongly to apatite and calcium and is thought to play a role in mineralisation and calcium ion homeostasis.
Purified protein from bovine bone - liquid
>98% by SDS PAGE
Approx. Protein Concentrations
Total protein concentration 1.0 mg/ml
Reagents In The Kit
Preparing The Antibody
Phosphate buffered saline
Store at -20oC only. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
1. Funaoka, H. et al. (2010) Development of a high-specificity enzyme-linked immunosorbent assay (ELISA) system for the quantification and validation of intact rat osteocalcin.Immunol Invest. 39: 54-73.