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Neurofilament H Antibody

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  • Rabbit anti Bovine Neurofilament H 200kDa
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP2259GAP *, WBdatasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Rabbit anti Bovine Neurofilament H 200 kDa antibody recognizes bovine intermediate 200 kDa Neurofilament H (NFH), a 1026 amino acid ~200 kDa protein involved in the maintenence of neuronal integrity.

      Neurofilaments are a major component of the cellular cytoskeleton, acting as the most abundant support for the axon cytoplasm. They exist as three neurofilament proteins: 68/70 kDa (NFL) 160 kDa (NFM) and 200 kDa (NFH), which are expressed in both the central and peripheral nervous systems. Although typically expressed by neurons, neurofilaments are also expressed by neuroblastomas, neuromas, gangliogliomas, ganglioneuromas, and ganglioneuroblastomas, as well as paragangliomas, carcinoids, skin neuroendocrine carcinomas, and oat cell carcinomas of the lung (Lehto et al. 1983).

      Defects in NFH are responsible for susceptibility to the neurodegenerative disorder amyotrophic lateral sclerosis (ALS) which affects upper and lower motor neurons, and results in fatal paralysis (Mendonça et al. 2005).
    • Intended Use
    • Target Species
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antiserum to bovine NFH was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
    • Preservative Stabilisers
      0.05% Sodium Azide (NaN3)
    • Immunogen
      GST-tagged bovine NFH recombinant protein.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      TRIS-glycine buffered saline, NaCl
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      microtubule cytoskeleton organization
      neurofilament cytoskeleton organization
      nervous system development
      intermediate filament bundle assembly
      cell death
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting0.1ug/ml
      Immunohistology - Paraffin(1)1.0ug/ml
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
      Bovine or rat cerebellum
    • Immunofluorescence
    • Western Blotting
      AHP2259GA detects a band of approximately 200kDa in bovine cerebellum tissue lysates.
    • Instructions For Use

    Additional Neurofilament H Antibody Formats

    Formats Applications Sizes available
    Neurofilament H Antibody : Purified P *, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (Fc):BiotinSTAR121B1 mgE, WB
      Sheep anti Rabbit IgG:Biotin2AB02B1 mlC, E, P, WB
      Sheep anti Rabbit IgG:DyLight®488STAR36D488GA0.1 mgF, IF
      Sheep anti Rabbit IgG:DyLight®549STAR36D549GA0.1 mgF, IF
      Sheep anti Rabbit IgG:DyLight®649STAR36D649GA0.1 mgF, IF
      Sheep anti Rabbit IgG:Dylight®800STAR36D800GA0.1 mgF, IF, WB
      Goat anti Rabbit IgG (Fc):FITCSTAR121F1 mgF
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      Sheep anti Rabbit IgG:RPESTAR35A1 mlF

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          TidyBlot Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP

          Recommended Positive Controls

            Histology Controls

              Bovine or rat cerebellum
              • Application NameReference Images
                Western Blotting


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