Mouse anti Human MBP antibody, clone 10 recognizes the synthetic peptide 82-91 and human and bovine 45-91 but not with whole native MBP molecule (Martensen numbering). This epitope is located in the C terminal region of 82-91 and the corresponding epitope is not present (or exposed) in whole MBP.
The clone was selected using synthetic human MBP peptide 82-91 for screening (Groome, N.P. et al 1985). Since this work was done a sequence error has been described in the human MBP sequence in this region (Gibson, et al. 1984).
Although the clone was selected to have a high affinity for 82-91 it appears that this antibody reacts more strongly with the unnatural sequence, rather than the correct human 82-91. In consequence the antibody is not a suitable reagent for sensitive immunoassays of correct human 82-91 as was thought at the time the original paper on this antibody was published.
The 91-92 phe-phe bond is believed to be cleaved by demyelinating lesions by Cathepsin D to generate peptides ending in the phe 91 which should react with this monoclonal.
Ascites fluid - liquid
Bovine Myelin Basic protein (MBP) (45-91) / Ovalbumin Conjugate
Approx. Protein Concentrations
Reagents In The Kit
Preparing The Antibody
Spleen cells from immunised A/J mice were fused with cells of the NS0 mouse myeloma cell line.
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Immunohistology - Frozen
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
3. Gibson, B.W., Gilliom, R.D., Whitaker, J.N. and Bieman, K. (1984) Amino acid sequence of human myelin basic protein peptide 45-89 as determined by mass spectrometry.J. Biol. Chem. 259: 5028-5031.
4. Sakuma, H. et al. (2006) Quantitation of myelin oligodendrocyte glycoprotein and myelin basic protein in the thymus and central nervous system and its relationship to the clinicopathologic features of autoimmune encephalomyelitis.J Neurosci Res. 84: 606-13.