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MBP Antibody | 10

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  • Mouse anti MBP (aa82-91)
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
  • Isotype
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA185C, E, WBdatasheet pdfdatasheet pdf0.25 ml
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Mouse anti Human MBP antibody, clone 10 recognizes the synthetic peptide 82-91 and human and bovine 45-91 but not with whole native MBP molecule (Martensen numbering). This epitope is located in the C terminal region of 82-91 and the corresponding epitope is not present (or exposed) in whole MBP.

      The clone was selected using synthetic human MBP peptide 82-91 for screening (Groome, N.P. et al 1985). Since this work was done a sequence error has been described in the human MBP sequence in this region (Gibson, et al. 1984).

      Although the clone was selected to have a high affinity for 82-91 it appears that this antibody reacts more strongly with the unnatural sequence, rather than the correct human 82-91. In consequence the antibody is not a suitable reagent for sensitive immunoassays of correct human 82-91 as was thought at the time the original paper on this antibody was published.

      The 91-92 phe-phe bond is believed to be cleaved by demyelinating lesions by Cathepsin D to generate peptides ending in the phe 91 which should react with this monoclonal.
    • Intended Use
    • Target Species
    • Product Form
      Ascites fluid - liquid
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
      0.09%Sodium Azide
    • Immunogen
      Bovine Myelin Basic protein (MBP) (45-91) / Ovalbumin Conjugate
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
    • Fusion Partners
      Spleen cells from immunised A/J mice were fused with cells of the NS0 mouse myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      protein binding
      plasma membrane
      structural constituent of myelin sheath
      myelin sheath
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen1/100
      Western Blotting

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional MBP Antibody Formats

    Formats Clone Applications Sizes available
    MBP Antibody : Ascites 10 C, E, WB 0.25 ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls


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