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Intestinal Alkaline Phosphatase Antibody

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Published customer image:
Rabbit anti intestinal alkaline phosphatase antibody used for western blotting
Image caption:
DRM analysis of membrane-associated FM. Total membranes (TMs) and DRMs were prepared from mucosal explants cultured for 1 h in the presence of FM (A) or from microvillar membrane vesicles treated with FM (B), as described in Methods. 5 μl (A) -or 2 μl (B) samples of serial dilutions of the membrane fractions were spotted onto Whatman filters and images captured under uv-light. FM was detected in TMs of both mucosal explants and microvillar membrane vesicles, but only in DRMs of the latter. C: SDS/PAGE of TMs and DRMs prepared as described in Methods from either total mucosal- or microvillar membranes. Samples of 25 μl were applied to each well. 1: Mucosal TMs; 2: Mucosal DRMs; 3: Microvillar TMs; 4: Microvillar DRMs. After electrophoresis and transfer onto a PVDF membrane, the lipid raft marker alkaline phosphatase (AP, 67 kDa) and lactase/phlorizin hydrolase (LPH, 160 kDa), a non-raft marker, were visualized by immunoblotting. Total protein was stained with Coomassie brilliant blue. Molecular mass values (kDa) are indicated by arrows.

From: Danielsen EM, Hansen GH (2013) Generation of Stable Lipid Raft Microdomains in the Enterocyte Brush Border by Selective Endocytic Removal of Non-Raft Membrane. PLoS ONE 8(10): e76661.

  • Intestinal Alkaline Phosphatase Antibody thumbnail image 1
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    0300-1024GE, WBdatasheet pdfdatasheet pdf1 ml
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Rabbit anti Calf alkaline phosphatase polyclonal antibody recognizes calf intestinal alkaline phosphatase- (IAP), a membrane-bound hydrolase enzyme highly expressed by intestinal mucosa (Hansen et al. 2011).

      Alkaline phosphatase (AP) is a ubiquitously expressed enzyme which removes phosphate groups from target molecules, including DNA, RNA and alkaloids, under alkaline conditions, and is present at higher concentrations in the placenta (placental AP), intestines (intestinal AP) and liver/bone/kidney (tissue non-specific AP). In vivo studies have identified CIAP as a potential therapeutic agent for the treatment of lipopolysaccharide (LPS)-mediated inflammation and sepsis, due to the ability of IAP to neutralise and thereby detoxify LPS (Peters et al. 2013).
    • Intended Use
    • Target Species
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to calf alkaline phosphatase were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
    • Preservative Stabilisers
      0.09%Sodium Azide (NaN3)
    • Immunogen
      Highly pure calf intestinal alkaline phosphatase.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 5.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      alkaline phosphatase activity
      plasma membrane
      integral to membrane
      anchored to membrane
      metal ion binding
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      0300-1024G detects a band of approximately 67kDa in porcine microvillar membranes (see reference 1).
    • Instructions For Use

    Additional Intestinal Alkaline Phosphatase Antibody Formats

    Formats Applications Sizes available
    Intestinal Alkaline Phosphatase Antibody : Purified E, WB 1 ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (Fc):BiotinSTAR121B1 mgE, WB
      Sheep anti Rabbit IgG:Biotin2AB02B1 mlC, E, P, WB
      Sheep anti Rabbit IgG:DyLight®488STAR36D488GA0.1 mgF, IF, WB
      Sheep anti Rabbit IgG:DyLight®549STAR36D549GA0.1 mgF, IF
      Sheep anti Rabbit IgG:DyLight®649STAR36D649GA0.1 mgF, IF
      Sheep anti Rabbit IgG:DyLight®680STAR36D680GA0.1 mgF, IF, WB
      Sheep anti Rabbit IgG:Dylight®800STAR36D800GA0.1 mgF, IF, WB
      Goat anti Rabbit IgG (Fc):FITCSTAR121F1 mgF
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      Sheep anti Rabbit IgG:RPESTAR35A1 mlF

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          TidyBlot™ Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*

          Recommended Positive Controls

            Histology Controls

                • Product Images

                • Intestinal Alkaline Phosphatase Antibody thumbnail image 1
              • Application NameReference Images
                Western Blotting


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