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IL-1 Alpha

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Bovine Interleukin-1 alpha ELISA

Recombinant Bovine interleukin-1 alpha(PBP031) detected using Rabbit anti Bovine interleukin-1 alpha (AHP2373) as the capture reagent and Rabbit anti Bovine interleukin-1 alpha:Biotin (AHP2373B) as the detection reagent followed by Streptavidin:HRP

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  • Recombinant Bovine Interleukin-1 Alpha
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  • Product Type
    Recombinant Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PBP031E, FNdatasheet pdfdatasheet pdf5 µg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Recombinant Bovine interleukin-1 alpha (IL-1α) is a recombinant protein corresponding to aa 120-268 of native bovine IL-1α.

      Interleukin-1α is a pleiotropic pro-inflammatory cytokine and member of the IL-1 superfamily. It is secreted by activated macrophages and monocytes and is involved in the modulation of a wide range of immune and inflammatory responses.

      IL-1α and the closely related IL-1β, are both involved in haematopoiesis and exhibit many identical immunomodulatory properties, binding to the same type I interleukin-1 receptor, also known as CD121a.
    • Intended Use
    • Target Species
    • Product Form
      Purified recombinant protein - lyophilized
    • Reconstitution
      Reconstitute with 20 µl sterile PBS containing 0.1% carrier protein
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
    • Preparation
      Recombinant protein expressed in Pichia pastoris and purified by ion-exchange chromatography
    • Preservative Stabilisers
      10% Trehalose
    • Purity
      >95% by SDS PAGE analysis
    • Approx. Protein Concentrations
    • Protein Molecular Weight
      17.1 kDa
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Prior to reconstitution store at -20oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.
    • Shelf Life
      12 months from date of despatch
    • GO Terms
      extracellular space
      interleukin-1 receptor binding
      cellular response to heat
      response to copper ion
      immune response
      copper ion binding
      positive regulation of cell division
      fever generation
      cytokine activity
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Functional Assays

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
      This reagent may be used as a standard in a sandwich ELISA assay in conjunction with AHP2373 as a coating antibody and AHP2373B as a detection antibody.
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional IL-1 Alpha Formats

    Formats Applications Sizes available
    IL-1 Alpha : Rec. Protein E, FN 5 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

                • Product Images

                • IL-1 Alpha thumbnail image 1

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