IgG1

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ELISA detection of immobilized, purified bovine IgG1 (PBP003) detected with Mouse anti Bovine IgG1 (MCA627GA) followed by Goat anti Mouse IgG:FITC (STAR117F)

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  • Purified Bovine IgG1
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Purified Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PBP003Edatasheet pdfdatasheet pdf1 mg
    PBP003
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • This product is a preparation of purified polyclonal bovine IgG1 prepared from normal bovine serum.
      By immunoelectrophoresis, PBP003 was shown to react with bovine IgG1. No reactivity was observed with antiserum specific for bovine IgG2, IgA or IgM.

      PBP003 contained <1% IgG2, IgA and IgM as determined by ELISA.
    • Intended Use
    • Target Species
      Bovine
    • Product Form
      Purified IgG1 - liquid
    • Reconstitution
    • Preparation
      Purified IgG1 prepared by ion exchange chromatography
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional IgG1 Formats

    Formats Applications Sizes available
    IgG1 : Purified E 1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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