IgA Antibody

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Biotin conjugated Sheep anti Bovine IgA antibody (AAI20B) used for the detection of M. avium subsp. paratuberculosis protein specific IgG secreted by cells from infected calves by ELISPOT along with biotinylated Sheep anti Bovine IgG antibody (AAI23B ) used to evaluate similar IgG responses.
Image caption:
Net IgA-ASC and IgG-ASC responses to recombinant M. avium subsp. paratuberculosis proteins. Cell suspensions were prepared from PP collected from M. avium subsp. paratuberculosis-infected and uninfected compartments in mid-jejunal segments and assayed for IgA (A) and IgG (B) ASCs specific to recombinant M. avium subsp. paratuberculosis proteins. Cell suspensions were prepared from PP collected from M. avium subsp. paratuberculosis-infected and uninfected compartments in terminal small intestinal segments and assayed for IgG (C) ASCs specific to recombinant M. avium subsp. paratuberculosis proteins. The net number of ASC specific to each M. avium subsp. paratuberculosis protein in infected compartments was calculated using the formula provided in Materials and Methods. M. avium subsp. paratuberculosis proteins for which all calves (n = 3) had detectable net ASC responses are shown with significant (P <0.05) increases in the frequency of M. avium subsp. paratuberculosis-protein specific ASCs denoted by an asterisk. Data presented are mean values with SEM.

From: Facciuolo A, Gonzalez-Cano P, Napper S, Griebel PJ, Mutharia LM (2016)
Marked Differences in Mucosal Immune Responses Induced in Ileal versus Jejunal Peyer's Patches to Mycobacterium avium subsp. paratuberculosis Secreted Proteins following Targeted Enteric Infection in Young Calves.
>PLoS ONE 11(7): e0158747.

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  • Sheep anti Bovine IgA
  • Sheep anti Bovine IgA:Alk. Phos.
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AAI20ABC, E, WBdatasheet pdfdatasheet pdf0.5 mg
    AAI20AB
    AAI20E, IDdatasheet pdfdatasheet pdf1 mg
    AAI20
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Sheep anti Bovine IgA antibody recognizes bovine IgA and shows no cross - reactivity with other bovine immunoglobulin classes in immunoelectrophoresis.
    • Intended Use
    • Target Species
      Bovine
    • Product Form
      Purified IgG - liquid
      Purified IgG conjugated to Alkaline Phosphatase - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to bovine IgA were raised by repeated immunisation of sheep with highly purified antigen. Purified IgG prepared by affinity chromatography.
    • Preservative Stabilisers
      0.09%Sodium Azide
      0.09% Sodium Azide (NaN3)
      0.2% Bovine Serum Albumin
    • Immunogen
      Purified bovine IgA.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
      IgG concentration 0.5mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      50mM HEPES
      0.1M NaCl
      1mM MgCl2
      0.1mM ZnCl2
    • Storage
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC.
      DO NOT FREEZE.

      This product should be stored undiluted.
      Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      12 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/1001/1000
      Immunodiffusion
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/10001/10000
      Immunohistology - Frozen1/1001/1000
      Western Blotting1/10001/10000

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional IgA Antibody Formats

    Formats Applications Sizes available
    IgA Antibody : Alk. Phos. C, E, WB 0.5 mg
    IgA Antibody : Purified E, ID 1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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