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Recombinant Bovine interferon &gamma used to stimulate bovine endothelial luteal cells in vitro prior to metabolite analysis by PCR and western blotting.
mRNA and protein expression for selected aa metabolites in non- and cytokine treated EnCL-1 cells. LTC4 synthase, LTA4 hydrolase, PGE2 synthase, PGF2α synthase and EDN-1 (A) mRNA, (B) protein expression of non-treated and cytokine (TNFα/IFNγ) stimulated EnCL-1 cells and (C) representive immunoblots of studies factors: 1 lane: Positive Control-bovine lung tissue, 2 lane-Control, 3 lane-TNFα/IFNγ. Arrows indicate statistical differences in the respective mRNA quantitative expression between groups of non-treated and cytokine treated cells (P<0.05), as determined by one-way ANOVA followed by t-student's multiple comparison test.
From: Korzekwa AJ, Bodek G, Bukowska J, Blitek A, Skarzynski DJ.
Characterization of bovine immortalized luteal endothelial cells: action of cytokines on production and content of arachidonic acid metabolites.
Reprod Biol Endocrinol. 2011 Feb 24;9:27.