IFN Gamma

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IFN Gamma gallery image 1

Published customer image:
Recombinant Bovine interferon &gamma used to stimulate bovine endothelial luteal cells in vitro prior to metabolite analysis by PCR and western blotting.
Image caption:
mRNA and protein expression for selected aa metabolites in non- and cytokine treated EnCL-1 cells. LTC4 synthase, LTA4 hydrolase, PGE2 synthase, PGF synthase and EDN-1 (A) mRNA, (B) protein expression of non-treated and cytokine (TNFα/IFNγ) stimulated EnCL-1 cells and (C) representive immunoblots of studies factors: 1 lane: Positive Control-bovine lung tissue, 2 lane-Control, 3 lane-TNFα/IFNγ. Arrows indicate statistical differences in the respective mRNA quantitative expression between groups of non-treated and cytokine treated cells (P<0.05), as determined by one-way ANOVA followed by t-student's multiple comparison test.

From: Korzekwa AJ, Bodek G, Bukowska J, Blitek A, Skarzynski DJ.
Characterization of bovine immortalized luteal endothelial cells: action of cytokines on production and content of arachidonic acid metabolites.
Reprod Biol Endocrinol. 2011 Feb 24;9:27.

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  • Recombinant Bovine Interferon Gamma
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  • Product Type
    Recombinant Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PBP007AE, FNdatasheet pdfdatasheet pdf25 µg
    PBP007A
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Recombinant Bovine interferon gamma, (IFNγ), known previously as macrophage activating factor, belongs to the type II interferon family and is produced by T lymphocytes and NK cells.

      Native bovine interferon gamma is a 143 amino acid cytokine with potent activating, anti-viral and anti-proliferative properties. It is produced as a pro-peptide with an additional 23 amino acid N-terminal signal peptide sequence having a molecular weight of ~20 kDa.

      IFNγ is predominantly secreted by activated T lymphocytes in response to specific mitogens as a result of infection and has numerous immunological regulatory functions. Properties include macrophage activation, upregulation of MHC class I and II molecules on various cells and induction of cytotoxicity.
    • Intended Use
    • Target Species
      Bovine
    • Product Form
      Purified recombinant protein - lyophilized
    • Reconstitution
      Reconstitute with 100 µl sterile PBS containing 0.1% carrier protein
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
    • Preparation
      Recombinant protein expressed in Pichia pastoris and purified by ion-exchange chromatography
    • Preservative Stabilisers
      10% Trehalose
    • Purity
      >95% by SDS PAGE analysis
    • Approx. Protein Concentrations
    • Protein Molecular Weight
      16.9 kDa
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Prior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted.
    • Shelf Life
      12 months from date of despatch
    • GO Terms
      response to virus
      cytokine activity
      interferon-gamma receptor binding
      extracellular space
      immune response
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Functional Assays

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
      This reagent has been used as a standard in a sandwich ELISA assay in conjunction with clone CC330 (MCA2112) and clone CC302 (MCA1783B)
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional IFN Gamma Formats

    Formats Applications Sizes available
    IFN Gamma : Rec. Protein E, FN 25 µg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse anti Bovine Interferon Gamma:BiotinMCA1783B0.25 mgE
          MCA1783B
          Mouse anti Bovine Interferon GammaMCA21120.5 mgE, ES, F *
          MCA2112

          Recommended Positive Controls

            Histology Controls

              References

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