IFN Gamma

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  • Recombinant Bovine Interferon Gamma
  • Recombinant Bovine Interferon Gamma
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Recombinant Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PBP007E, FNdatasheet pdfdatasheet pdf20 µg
    PBP007
    PBP007AE, FNdatasheet pdfdatasheet pdf25 µg
    PBP007A
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Recombinant Bovine interferon gamma, (IFNγ), known previously as macrophage activating factor, belongs to the type II interferon family and is produced by T lymphocytes and NK cells.

      Native bovine interferon gamma is a 143 amino acid cytokine with potent activating, anti-viral and anti-proliferative properties. It is produced as a pro-peptide with an additional 23 amino acid N-terminal signal peptide sequence having a molecular weight of ~20 kDa.

      IFNγ is predominantly secreted by activated T lymphocytes in response to specific mitogens as a result of infection and has numerous immunological regulatory functions. Properties include macrophage activation, upregulation of MHC class I and II molecules on various cells and induction of cytotoxicity.
    • Intended Use
    • Target Species
      Bovine
    • Product Form
      Pack Size: 20 µgPurified Recombinant Protein- liquid
      Pack Size: 25 µgPurified recombinant protein - lyophilized
      Pack Size: 20 µgPurified Recombinant Protein- liquid
      Pack Size: 25 µgPurified recombinant protein - lyophilized
    • Reconstitution
      Pack Size: 25 µgReconstitute with 100 µl sterile PBS containing 0.1% carrier protein
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
      Pack Size: 25 µgReconstitute with 100 µl sterile PBS containing 0.1% carrier protein
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
    • Preparation
      Pack Size: 20 µgRecombinant protein expressed in Pichia pastoris
      Pack Size: 25 µgRecombinant protein expressed in Pichia pastoris and purified by ion-exchange chromatography
      Pack Size: 20 µgRecombinant protein expressed in Pichia pastoris
      Pack Size: 25 µgRecombinant protein expressed in Pichia pastoris and purified by ion-exchange chromatography
    • Preservative Stabilisers
      Pack Size: 20 µgNone present
      Pack Size: 25 µg10% Trehalose
      Pack Size: 20 µgNone present
      Pack Size: 25 µg10% Trehalose
    • Purity
      >95% by SDS PAGE analysis
      >95% by SDS PAGE analysis
    • Approx. Protein Concentrations
      Pack Size: 20 µg0.565 mg/ml
      Pack Size: 20 µg0.565 mg/ml
    • Protein Molecular Weight
      16.9kD
    • Reagents In The Kit
    • Preparing The Antibody
    • Endotoxin Level
      <0.05EU/ug
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Pack Size: 20 µgStore at -70oC.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 25 µgPrior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted.
      Pack Size: 20 µgStore at -70oC.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 25 µgPrior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted.
    • Shelf Life
      12 months from date of despatch.
      12 months from date of despatch
    • GO Terms
      extracellular space
      interferon-gamma receptor binding
      cytokine activity
      response to virus
      immune response
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Functional Assays
    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Functional Assays

    • Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
      PBP007 has been used as a standard in a sandwich ELISA assay in conjunction with clone CC330 (MCA2112) and clone CC302 (MCA1783B)
    • ELISA
      This reagent has been used as a standard in a sandwich ELISA assay in conjunction with clone CC330 (MCA2112) and clone CC302 (MCA1783B)
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional IFN Gamma Formats

    Formats Applications Sizes available
    IFN Gamma : Rec. Protein E, FN 20 µg | 25 µg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse anti Bovine Interferon Gamma:BiotinMCA1783B0.25 mgE
          MCA1783B
          Mouse anti Bovine Interferon GammaMCA21120.5 mgE, ES, F *
          MCA2112

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Functional Assays

              References

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