IFN Gamma

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Typical assay curve using standards and reagents supplied in the Bovine IFN gamma ELISA kit (MCA5638KZZ)

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Published customer image:
Bovine interferon-γ kit used for the determination of interferon-γ in sheep and goat serum samples.
Image caption:
Quantification of interferon-gamma (IFN-γ) in serum samples collected from sheep and goats following PPRV infection. Measurement of IFN-γ levels were performed using an IFN-γ ELISA and cross-reactive antibodies against bovine IFN-γ, and quantified using a standard provided by the manufacturer. Results presented are the mean values with standard deviations from animals at each time point. P<0.05 for goats at 8 dpi compared to -2 dpi by t-test.

From: Truong T, Boshra H, Embury-Hyatt C, Nfon C, Gerdts V, et al. (2014) Peste des Petits Ruminants Virus Tissue Tropism and Pathogenesis in Sheep and Goats following Experimental Infection. PLoS ONE 9(1): e87145.

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Published customer image:
Bovine interferon gamma ELISA kit used for the measurement of interferon gamma levels in bronchoalveolar lavage from calves infected with bovine respiratory syncytial virus.
Image caption:
Variations of cells populations and cytokines in bronchoalveolar lavage (BAL) from calves challenged with BRSV-Snk. Experimental challenge by inhalation of aerosolized BRSV passaged in gnotobiotic calves (BRSV-Snk) was performed in five calves with moderate levels of BRSV-specific maternal antibodies (MDA). Samples of the cells populating the lower airways were collected via BAL seven days after infection, and in addition, from three healthy uninfected calves (Control). Cells in BAL samples were analyzed by light microscopy (panel A), and expressed as group mean ×106 cells/ml. The concentrations of indicated inflammatory cytokines in BAL sample supernatants were measured using specific ELISAs, and are expressed in ng/ml: (panel B) interleukin (IL)-4; (panel C) IL-6; (panel D) IL-8; (panel E) interferon gamma (IFN?); and (panel F) tumor necrosis factor alpha (TNFa). Individual data are indicated by rings and group means by horizontal lines. Probability (p) of statistically significant differences between groups is given in each panel, where p?=?0.05 was considered statistically significant.

From: A bovine respiratory syncytial virus model with high clinical expression in calves with specific passive immunity. Krister Blodörn, Sara Hägglund, Dolores Gavier-Widen, Jean-François Eléouët, Sabine Riffault, John Pringle, Geraldine Taylor, Jean Valarcher BMC Veterinary Research 2015, 11:76

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  • Bovine Interferon Gamma ELISA Kit
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Kits
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA5638KZZEdatasheet pdfdatasheet pdf datasheet pdf datasheet pdf datasheet pdf datasheet pdf5 X 96 Wells
    MCA5638KZZ
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • The Bovine Interferon Gamma Kit is specific for bovine interferon gamma (IFN-gamma), a secreted cytokine involved in anti-viral activity and growth regulation belonging to the type II interferon family.

      IFN-gamma is produced by lymphocytes in response to activation by specific antigens or mitogens. In addition to its anti-viral activity, IFN-gamma has important immunoregulatory functions in the activation of macrophages and anti-proliferative effects upon transformed cells.
    • Intended Use
      MCA5638KZZ is an ELISA kit (5 x 96 wells) designed for the quantitive determination of bovine interferon gamma (BoIFN-gamma) in bovine serum, plasma and culture supernatant.

      This assay can measure BoIFN-gamma in the range 0.025-50 ng/ml in bovine serum, plasma and culture supernatant.
    • Target Species
      Bovine
    • Product Form
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
      96 well microtiter plates x 5
      Microplate adhesive sealers x 5
      Coating antibody - 1vial (MCA5638KZZA)
      Coating Buffer - 1 vial (5 x concentrated)
      Detection antibody – Biotin conjugated - 1 vial (MCA5638KZZC)
      Streptavidin:HRP conjugate - 1 vial (MCA5638KZZD)
      TMB Substrate 1 vial
      Bovine IFN gamma standard (400ng/ml – Lyophilized) 1 vial (MCA5638KZZB)
    • Preparing The Antibody
    • Test Principle
      This assay is based on a sandwich ELISA using two different mouse anti bovine IFN-gamma monoclonal antibodies and a recombinant bovine IFN-gamma as a standard.
    • Buffer Solution
    • Additional Reagents Required
      The following additional reagents and Equipment are also required for successful use of this kits BUT ARE NOT CONTAINED WITHIN THIS KIT

      Wash buffer (available from Bio-Rad, Catalogue code BUF031A/B/C, or see kit insert for recipe)
      Blocking solution (available from Bio-Rad, Catalogue Code: BUF032A/B/C, or see kit insert for recipe)
      Stop Solution (see kit insert for recipe)
      Distilled or Deionised water
      Plate reader with 450nm reading capacity
      Pipettes for dispensing up to 250ul
      Tubes for preparing standard and sample dilutions
    • Storage
      Prior to use store all kit components at +4oC. DO NOT FREEZE. Please view the handbook for further details.
      Storage in frost free freezers is not recommended. This Kit contains components that are photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature components within this kit.
      The contents of this kit should be stored undiluted. Should components within this kit contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      Stored correctly, 18 months from date of despatch.
    • GO Terms
      cytokine activity
      immune response
      extracellular space
      interferon-gamma receptor binding
      response to virus
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      This kit and its components are Research Use Only. Not for Therapeutic or Diagnostic use.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use
      Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/MCA5638KZZ.pdf

    Additional IFN Gamma Formats

    Formats Applications Sizes available
    IFN Gamma : Kit E 5 X 96 Wells
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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