IFN Gamma Antibody | 5D10

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  • Mouse anti Bovine Interferon Gamma
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    5D10
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2689GAE, ESdatasheet pdfdatasheet pdf0.1 mg
    MCA2689GA
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Bovine Interferon Gamma (IFNγ), clone 5D10, recognises the 19.3kDa protein belonging to the type II interferon family .

      Bovine IFNγ is produced by activated lymphocytes, has anti-viral and immunoregulatory properties and is an activator of macrophages. Clone 5D10 is one of a range of bovine IFNγ reagents available from Bio-Rad which include the monoclonal antibody clones CC330, CC302 and 7B6 along with recombinant bovine IFNγ and bovine IFNγ ELISA kits.

      Clone 5D10 is suitable for ELISA and ELISpot, for intracellular identification of bovine IFNγ and may be used in conjuction with clone CC302 (clone CC302 is available in a range of direct conjugates) as a detection reagent.
    • Intended Use
    • Target Species
      Bovine
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Purified recombinant bovine IFN gamma expressed in E.coli.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised Balb/c mice were fused with cells of the Sp20/Ag-14 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      response to virus
      cytokine activity
      interferon-gamma receptor binding
      immune response
      extracellular space
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      ELISpot

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
      This product may be used in an indirect ELISA or as a capture antibody in a sandwich ELISA together with MCA1783B as the detection reagent. PBP007 may be used as a standard.
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional IFN Gamma Antibody Formats

    Formats Clone Applications Sizes available
    IFN Gamma Antibody : Purified 5D10 E, ES 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
      STAR117A
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      STAR87A
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
      STAR117D488GA
      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF
      STAR117D549GA
      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      STAR117D649GA
      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
      STAR117D680GA
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      STAR117D800GA
      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      STAR8D800GA
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      STAR117F
      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
      STAR70
      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
      STAR120F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      STAR9B
      Human anti Mouse IgG1:HRPHCA036P0.1 mgE
      HCA036P
      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      STAR117P
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      STAR77
      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
      STAR120P
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
      STAR13B
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      STAR87P
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      STAR12A
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
      STAR76

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse anti Bovine Interferon Gamma:Alexa Fluor®488MCA1783A488100 Tests/1mlF *
          MCA1783A488
          Mouse anti Bovine Interferon Gamma:Alexa Fluor®647MCA1783A647100 Tests/1mlF *
          MCA1783A647
          Mouse anti Bovine Interferon Gamma:BiotinMCA1783B0.25 mgE
          MCA1783B
          Mouse anti Bovine Interferon Gamma:FITCMCA1783F0.1 mgF *
          MCA1783F
          Bovine Interferon Gamma ELISA KitMCA5638KZZ5 X 96 WellsE
          MCA5638KZZ
          Mouse anti Bovine Interferon GammaMCA19640.5 mgF *, P *, WB
          MCA1964
          Mouse anti Bovine Interferon GammaMCA21120.5 mgE, ES, F *
          MCA2112
          Recombinant Bovine Interferon GammaPBP00720 µgE, FN
          PBP007
          Mouse anti Bovine Interferon Gamma:RPEMCA1783PE100 TestsF *
          MCA1783PE

          Recommended Positive Controls

            Histology Controls

              References

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