Glutathione Peroxidase Antibody

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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    4690-3999GEdatasheet pdfdatasheet pdf1 ml
    4690-3999G
    4690-3999AEdatasheet pdfdatasheet pdf1 ml
    4690-3999A
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Sheep anti Bovine glutathione peroxidase antibody detects human glutathione peroxidise (GSH-Px), a selenium-containing tetrameric enzyme found in mammalian erythrocytes. GSH-Px has an important role in protecting cellular and subcellular membranes from oxidative damage, and functions to detoxify peroxide though the reduction of hydrogen peroxide and organic peroxides.

      An increase in GSH-Px in tumour cells may be associated with resistance to drugs that act by generating reactive oxygen species, for example doxorubicin.
    • Intended Use
    • Target Species
      Bovine
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      HumanExpected from Sequence
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to bovine glutathione peroxidase were raised by repeated immunisations of sheep with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
    • Preservative Stabilisers
      0.09%Sodium Azide (NaN3)
      0.09%Sodium Azide (NaN3)
      1%Bovine Serum Albumin
    • Immunogen
      Glutathione peroxidase from bovine erythrocytes.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 5.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      cytoplasm
      response to oxidative stress
      glutathione peroxidase activity
      oxidation-reduction process
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/1001/1000
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/1001/250

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional Glutathione Peroxidase Antibody Formats

    Formats Applications Sizes available
    Glutathione Peroxidase Antibody : Alk. Phos. E 1 ml
    Glutathione Peroxidase Antibody : Purified E 1 ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

              Further Reading

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