Gelsolin

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  • Native Bovine Gelsolin
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Purified Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PBP013FNdatasheet pdfdatasheet pdf20 µg
    PBP013
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Gelsolin is a calcium and polyphosphoinositide regulated actin-binding protein which consists of 6 homologous domains. It is involved in cell morphology, motility, growth and apoptosis. It affects filament assembly by severing actin filaments, forming a nucleus for polymerisation, and capping the fast exchanging end of the filaments.
    • Intended Use
    • Target Species
      Bovine
    • Product Form
      Purified protein from bovine plasma - lyophilised
    • Reconstitution
      Use sterile distilled water to give a concentrated stock solution => 1 mg/ml.
    • Preparation
    • Preservative Stabilisers
      See Buffer solution.
    • Activity
      54 U/mg protein.
      One unit will reduce the viscosity difference between an actin solution and buffer by 50% in a 1 ml reaction mixture containing 1-2 mg F-actin, 0.15 M KCl, 20 mM TRIS, pH 7.6, 0.2 mM CaCl2, 0.2 mM ATP and 1 mM DTT at 28ºC.
    • Purity
      SDS PAGE: >95%
    • Approx. Protein Concentrations
      5%, remainder buffer salts.
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Tris buffered saline, EGTA
    • Storage
      Prior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein.
    • Shelf Life
      12 months from date of despatch.
    • GO Terms
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Functional Assays

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Gelsolin Formats

    Formats Applications Sizes available
    Gelsolin : Purified FN 20 µg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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