Gelsolin is a calcium and polyphosphoinositide regulated actin-binding protein which consists of 6 homologous domains. It is involved in cell morphology, motility, growth and apoptosis. It affects filament assembly by severing actin filaments, forming a nucleus for polymerisation, and capping the fast exchanging end of the filaments.
Purified protein from bovine plasma - lyophilised
Use sterile distilled water to give a concentrated stock solution => 1 mg/ml.
See Buffer solution.
54 U/mg protein.One unit will reduce the viscosity difference between an actin solution and buffer by 50% in a 1 ml reaction mixture containing 1-2 mg F-actin, 0.15 M KCl, 20 mM TRIS, pH 7.6, 0.2 mM CaCl2, 0.2 mM ATP and 1 mM DTT at 28ºC.
SDS PAGE: >95%
Approx. Protein Concentrations
5%, remainder buffer salts.
Reagents In The Kit
Preparing The Antibody
Tris buffered saline, EGTA
Prior to reconstitution store at +4oC. After reconstitution store at -20oC. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.