Fetuin, a glycoprotein present in serum, is synthesized by hepatocytes and is an important inhibitor of soft tissue calcification. It is frequently used as a tissue culture medium supplement contributing to the attachment and spreading of cells. Fetuin is a member of the cystatin superfamily of proteins.
Purified bovine fetuin from fetal calf serum - Lyophilised
Use sterile distilled water to give a solution of 1 mg/ml.
Protein content: >80% Total impurities: <0.3% Free N-acetyl-neuraminic acid
Approx. Protein Concentrations
Reagents In The Kit
Preparing The Antibody
Prior to reconstitution store at +4oC. After reconstitution store at -20oC. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
1. Dziegielewska, K. M. et al. (1990) The complete cDNA and amino acid sequence of bovine fetuin. Its homology with alpha 2HS glycoprotein and relation to other members of the cystatin superfamily.J. Biol. Chem. 265: 4354 - 4357.