Collagen I/III Antibody

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  • Rabbit anti Bovine Collagen I/III
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    2150-2305E, IFdatasheet pdfdatasheet pdf0.5 ml
    2150-2305
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • 2150-2305 reacts with both Collagen I and III. Less than 0.5% reactivity was observed with bovine albumin and immunoglobulins.

      Collagen is located in the extracellular matrix of connective tissues. It is part of the interacting network of proteoglycans and proteins that provides a structural framework for both soft and calcified connective tissues.
    • Intended Use
    • Target Species
      Bovine
    • Product Form
      Purified IgG - lyophilised
    • Reconstitution
      Use 500 µl sterile distilled water or saline.
    • Preparation
      Affinity chromatography on immobilized collagen I and III
    • Preservative Stabilisers
      1% Dextran
      1% Mannitol
    • Immunogen
      Bovine collagen type I and III from skin
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate/borate buffered saline
    • Storage
      Prior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.
    • Shelf Life
      12 months from date of reconstitution.
    • GO Terms
      proteinaceous extracellular matrix
      extracellular matrix structural constituent
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/2,0001/8,000
      Immunofluorescence1/101/40

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Collagen I/III Antibody Formats

    Formats Applications Sizes available
    Collagen I/III Antibody : Purified E, IF 0.5 ml
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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