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CD8a antibody | 7C2B

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Anti Bovine CD8a Antibody, clone 7C2B thumbnail image 1
Anti Bovine CD8a Antibody, clone 7C2B gallery image 1
Flow cytometric staining of bovine peripheral blood lymphocytes.
A. Mouse IgG2a negative control (MCA929) followed by Goat anti Mouse IgG2a:PE (STAR133PE).
B. Mouse anti Bovine CD8a antibody, clone 7C2B (MCA6083) followed by Goat anti Mouse IgG2a:PE secondary antibody (STAR133PE).
Both the Mouse IgG2a negative control and Mouse anti Bovine CD8a are plotted against bovine CD40 as recognized by FITC conjugated Mouse anti Bovine CD40 antibody, clone IL-A156 (MCA2431F).

Mouse anti Bovine CD8a

Product Type
Monoclonal Antibody
Clone
7C2B
Isotype
IgG2a
Specificity
CD8a

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Product Code Applications Pack Size List Price Your Price Qty
MCA6083
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
List Price Your Price
loader

Mouse anti Bovine CD8a, clone 7C2B, recognizes bovine CD8a. CD8a is a coreceptor for MHC I molecules. It is involved in the activation of the cytotoxic T-cell response and the clearance of virally infected cells. CD8 is predominantly present either as homodimer or a multimer of the alpha chain or as heterodimer of the alpha and beta chains (Littman et al. 1987). Bovine CD8 differs from the CD8 molecules of other animal species and shares 57.85% identity with swine, 37.70% with mouse, 27.50 % with chicken, and 20.69% identity with carp (Zhenbao et al. 2014).

Mouse anti Bovine CD8a, clone 7C2B recognizes bovine CD8 lymphocytes and gamma delta CD8+ lymphocytes (Park et al. 2000). In addition it reacts with the CD8 lymphocytes of water buffalo (Davis et al. 2001).

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Goat
Sheep
Bison
Water Buffalo
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Concentrated tissue culture supernatant - liquid
Preparation
Concentrated tissue culture supernatant clarified by filtration through a 0.2 micrometer filter
Buffer Solution
Serum free tissue culture medium containing proprietary protein free supplement
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at +4oC. DO NOT FREEZE.
Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Functional Assays
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG2a:RPE STAR133PE F 0.1 mg loader
List Price Your Price
loader
Description Goat anti Mouse IgG2a:RPE

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse anti Bovine CD3 MCA6080 F IC 0.1 mg
List Price Your Price
Description Mouse anti Bovine CD3
Mouse anti Bovine CD4 MCA6081 F 0.1 mg
List Price Your Price
Description Mouse anti Bovine CD4

References for CD8a antibody

  1. Koo H.C. et al. (2004) Analysis of the immune response to Mycobacterium avium subsp. paratuberculosis in experimentally infected calves.
    Infect Immun. 72 (12): 6870-83.
  2. Allen A.J. et al. (2009) Development of a bovine ileal cannulation model to study the immune response and mechanisms of pathogenesis of paratuberculosis.
    Clin Vaccine Immunol. 16 (4): 453-63.

UniProt
P31783
Entrez Gene
CD8A
GO Terms
GO:0016021 integral to membrane

MCA6083

142897

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