CD8a Antibody | 7C2B

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CD8a Antibody | 7C2B gallery image 1

Flow cytometric staining of bovine peripheral blood lymphocytes.
A. Mouse IgG2a negative control (MCA929) followed by Goat anti Mouse IgG2a:PE (STAR133PE).
B. Mouse anti Bovine CD8a antibody, clone 7C2B (MCA6083) followed by Goat anti Mouse IgG2a:PE secondary antibody (STAR133PE).
Both the Mouse IgG2a negative control and Mouse anti Bovine CD8a are plotted against bovine CD40 as recognized by FITC conjugated Mouse anti Bovine CD40 antibody, clone IL-A156 (MCA2431F).

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  • Mouse anti Bovine CD8a
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  • Product Type
    Monoclonal Antibody
  • Clone
    7C2B
  • Isotype
    IgG2a
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA6083Fdatasheet pdfdatasheet pdf0.1 mg
    MCA6083
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Bovine CD8a, clone 7C2B, recognizes bovine CD8a. CD8a is a coreceptor for MHC I molecules. It is involved in the activation of the cytotoxic T-cell response and the clearance of virally infected cells. CD8 is predominantly present either as homodimer or a multimer of the alpha chain or as heterodimer of the alpha and beta chains (Littman et al. 1987). Bovine CD8 differs from the CD8 molecules of other animal species and shares 57.85% identity with swine, 37.70% with mouse, 27.50 % with chicken, and 20.69% identity with carp (Zhenbao et al. 2014).

      Clone 7C2B recognizes bovine CD8 lymphocytes and gamma delta CD8+ lymphocytes (Park et al. 2000). In addition it reacts with the CD8 lymphocytes of water buffalo (Davis et al. 2001).
    • Intended Use
    • Target Species
      Bovine
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Goatyes
      Sheepyes
      Bisonyes
      Water Buffaloyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Concentrated tissue culture supernatant - liquid
    • Reconstitution
    • Preparation
      Concentrated tissue culture supernatant clarified by filtration through a 0.2 micrometer filter
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Serum free tissue culture medium containing proprietary protein free supplement
    • Storage
      Store at +4oC. DO NOT FREEZE.
      Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch
    • GO Terms
      integral to membrane
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
      Functional Assays

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD8a Antibody Formats

    Formats Clone Applications Sizes available
    CD8a Antibody : Con S/N 7C2B F 0.1 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG2a:RPESTAR133PE0.1 mgF
      STAR133PE

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse anti Bovine CD3MCA60800.1 mgF, IC
          MCA6080
          Mouse anti Bovine CD4MCA60810.1 mgF
          MCA6081

          Recommended Positive Controls

            Histology Controls

              References

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