Flow cytometric staining of bovine peripheral blood lymphocytes. A. Mouse IgG2a negative control (MCA929) followed by Goat anti Mouse IgG2a:PE (STAR133PE). B. Mouse anti Bovine CD8a antibody, clone 7C2B (MCA6083) followed by Goat anti Mouse IgG2a:PE secondary antibody (STAR133PE). Both the Mouse IgG2a negative control and Mouse anti Bovine CD8a are plotted against bovine CD40 as recognized by FITC conjugated Mouse anti Bovine CD40 antibody, clone IL-A156 (MCA2431F).
Mouse anti Bovine CD8a, clone 7C2B, recognizes bovine CD8a. CD8a is a coreceptor for MHC I molecules. It is involved in the activation of the cytotoxic T-cell response and the clearance of virally infected cells. CD8 is predominantly present either as homodimer or a multimer of the alpha chain or as heterodimer of the alpha and beta chains (Littman et al. 1987). Bovine CD8 differs from the CD8 molecules of other animal species and shares 57.85% identity with swine, 37.70% with mouse, 27.50 % with chicken, and 20.69% identity with carp (Zhenbao et al. 2014).
Clone 7C2B recognizes bovine CD8 lymphocytes and gamma delta CD8+ lymphocytes (Park et al. 2000). In addition it reacts with the CD8 lymphocytes of water buffalo (Davis et al. 2001).
N.B. Antibody reactivity and working conditions may vary between species.
Concentrated tissue culture supernatant - liquid
Concentrated tissue culture supernatant clarified by filtration through a 0.2 micrometer filter
0.09% Sodium Azide (NaN3)
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Reagents In The Kit
Preparing The Antibody
Serum free tissue culture medium containing proprietary protein free supplement
Store at +4oC. DO NOT FREEZE. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Use 10ul of the suggested working dilution to label 106 cells in 100ul