CD5 Antibody | CC17

CD5 Antibody | CC17 gallery image 1

Staining of bovine peripheral blood lymphocytes with Mouse anti Bovine CD5 (MCA835G)

CD5 Antibody | CC17 gallery image 2

Staining of bovine peripheral blood lymphocytes with Mouse anti Bovine CD5 (MCA835G) followed by Goat anti Mouse IgG (H/L):FITC (STAR117F)

  • CD5 Antibody | CC17 thumbnail image 1
  • CD5 Antibody | CC17 thumbnail image 2
  • Mouse anti Bovine CD5
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  • Product Type
    Monoclonal Antibody
  • Clone
  • Isotype
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA835GAC, F, IPdatasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Mouse anti Bovine CD5 antibody, clone CC15 recognizes bovine CD5, a ~67 kDa type 1 single pass transmembrane molecule containing three scavenger receptor cysteine rich (SRCR) domains. Clone CC17 reacts with BoCD5.1, which is a polymorphic antigen expressed on cells of all Bos taurus animals and a small proportion of Bos indicus animals.
      Bo5.1, recongnized by Mouse anti Bovine CD5, clone CC17 is the only isoform expressed by Bos taurus while Bos indicus may express Bo5.2 or both allelic forms of bovine CD5. (Howard et al.1989) .

      Bovine CD5 is expressed by all mature T-lymphocytes and a subpopulation of B-lymphocytes. It is also expressed by mature medullary thymocytes and at a lower level by immature cortical thymocytes (Howard et al. 1988).
    • Intended Use
    • Target Species
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Bovine thymocytes
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from an immunised mouse were fused with cells of the mouse NS1 myeloma cell line
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch
    • GO Terms
      scavenger receptor activity
      integral to membrane
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/251/100
      Immunohistology - Frozen

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD5 Antibody Formats

    Formats Clone Applications Sizes available
    CD5 Antibody : Purified CC17 C, F, IP 0.1 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Flow Cytometry


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