Flow cytometric staining of bovine peripheral blood lymphocytes. A. Mouse IgG2a negative control (MCA929) followed by Goat anti Mouse IgG2a:PE (STAR133PE). B. Mouse anti Bovine CD5 antibody, clone B29A (MCA6082) followed by Goat anti Mouse IgG2a:PE secondary antibody (STAR133PE). Both the Mouse IgG2a negative control and Mouse anti Bovine CD5 are plotted against bovine CD40 as recognized by FITC conjugated Mouse anti Bovine CD40 antibody, clone IL-A156 (MCA2431F).
Mouse anti Bovine CD5, clone B29A, recognizes bovine CD5. The 67 kDa glycoprotein CD5 is present on alpha beta and gamma delta T cells but only on a small number of B cells. In contrast, in cattle infected with bovine leukemia virus almost all B cells were CD5+ (Depelchin et al. 1989). CD5 associates with CD2 and the CD3 zeta chain (Burgess et al. 1992). Similarly to mouse or human, in cattle CD5 associates with the B cell receptor. However, in cattle infected with bovine leukemia virus CD5 dissociates from the B cell receptor (Cantor et al. 2001).
Concentrated tissue culture supernatant - liquid
Concentrated tissue culture supernatant clarified by filtration through a 0.2 micrometer filter
0.09% Sodium Azide (NaN3)
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Reagents In The Kit
Preparing The Antibody
Serum free tissue culture medium containing proprietary protein free supplement
Store at +4oC. DO NOT FREEZE. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Use 10ul of the suggested working dilution to label 106 cells in 100ul
1. Davis W.C. et al. (1987) The development and analysis of species specific and cross reactive monoclonal antibodies to leukocyte differentiation antigens and antigens of the major histocompatibility complex for use in the study of the immune system in cattle and other species.Vet Immunol Immunopathol. 15 (4): 337-76.