CD5 Antibody | B29A

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CD5 Antibody | B29A gallery image 1

Flow cytometric staining of bovine peripheral blood lymphocytes.
A. Mouse IgG2a negative control (MCA929) followed by Goat anti Mouse IgG2a:PE (STAR133PE).
B. Mouse anti Bovine CD5 antibody, clone B29A (MCA6082) followed by Goat anti Mouse IgG2a:PE secondary antibody (STAR133PE).
Both the Mouse IgG2a negative control and Mouse anti Bovine CD5 are plotted against bovine CD40 as recognized by FITC conjugated Mouse anti Bovine CD40 antibody, clone IL-A156 (MCA2431F).

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  • Mouse anti Bovine CD5
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  • Product Type
    Monoclonal Antibody
  • Clone
    B29A
  • Isotype
    IgG2a
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA6082Fdatasheet pdfdatasheet pdf0.1 mg
    MCA6082
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Bovine CD5, clone B29A, recognizes bovine CD5. The 67 kDa glycoprotein CD5 is present on alpha beta and gamma delta T cells but only on a small number of B cells. In contrast, in cattle infected with bovine leukemia virus almost all B cells were CD5+ (Depelchin et al. 1989). CD5 associates with CD2 and the CD3 zeta chain (Burgess et al. 1992).
      Similarly to mouse or human, in cattle CD5 associates with the B cell receptor. However, in cattle infected with bovine leukemia virus CD5 dissociates from the B cell receptor (Cantor et al. 2001).
    • Intended Use
    • Target Species
      Bovine
    • Product Form
      Concentrated tissue culture supernatant - liquid
    • Reconstitution
    • Preparation
      Concentrated tissue culture supernatant clarified by filtration through a 0.2 micrometer filter
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Bovine lymphocytes
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Serum free tissue culture medium containing proprietary protein free supplement
    • Storage
      Store at +4oC. DO NOT FREEZE.
      Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch
    • GO Terms
      scavenger receptor activity
      integral to membrane
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
      Functional Assays

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD5 Antibody Formats

    Formats Clone Applications Sizes available
    CD5 Antibody : Con S/N B29A F 0.1 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG2a:RPESTAR133PE0.1 mgF
      STAR133PE

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse anti Bovine CD3MCA60800.1 mgF, IC
          MCA6080

          Recommended Positive Controls

            Histology Controls

              References

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