Figure A. RPE conjugated mouse anti bovine CD28 (MCA5779PE) and FITC conjugated mouse IgG1 isotype control (MCA928F). Figure B. RPE conjugated mouse anti bovine CD28 (MCA5779PE) and FITC conjugated mouse anti bovine CD44 (MCA2433F). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.
Figure A. FITC conjugated mouse anti bovine CD44 (MCA2433F) and RPE conjugated mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated mouse anti bovine CD44 (MCA2433F) and RPE conjugated mouse anti bovine CD8b (MCA5779PE). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.
Mouse anti Bovine CD44 antibody, clone IL-A118 recognizes bocine Phagocytic Glycoprotein-1 (PGP-1), also known as CD44, Hermes antigen, Extracellular matrix receptor III or HUTCH-1. Bovine CD44 is a 346 amino acid ~90 kDa type I single pass transmembrane glycoprotein containing a single Link domain, responsible for hyaluronan binding. Bovine CD44 is expressed by a wide range of bovine cells, including peripheral T and B lymphocytes, monocytes, granulocytes, platelets and early erythroid cells.
N.B. Antibody reactivity and working conditions may vary between species.
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Bovine Serum Albumin
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Reagents In The Kit
Preparing The Antibody
Phosphate buffered saline
Spleen cells from immunised BALB/c mice were fused with cells of the X63.Ag8.853 myeloma cell line.
Store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul