CD44 Antibody | IL-A118

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Staining of Bovine peripheral blood lymphocytes with Mouse anti Bovine CD44 (MCA2433)

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Staining of Bovine peripheral blood lymphocytes with Mouse anti Bovine CD44:RPE (MCA2433PE)

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Staining of Bovine peripheral blood lymphocytes with Mouse anti Bovine CD44:FITC (MCA2433F)

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Figure A. RPE conjugated mouse anti bovine CD28 (MCA5779PE) and FITC conjugated mouse IgG1 isotype control (MCA928F). Figure B. RPE conjugated mouse anti bovine CD28 (MCA5779PE) and FITC conjugated mouse anti bovine CD44 (MCA2433F). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.

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Figure A. FITC conjugated mouse anti bovine CD44 (MCA2433F) and RPE conjugated mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated mouse anti bovine CD44 (MCA2433F) and RPE conjugated mouse anti bovine CD8b (MCA5779PE). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.

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  • Product Type
    Monoclonal Antibody
  • Clone
    IL-A118
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2433FFdatasheet pdfdatasheet pdf0.1 mg
    MCA2433F
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Bovine CD44 antibody, clone IL-A118 recognizes bocine Phagocytic Glycoprotein-1 (PGP-1), also known as CD44, Hermes antigen, Extracellular matrix receptor III or HUTCH-1. Bovine CD44 is a 346 amino acid ~90 kDa type I single pass transmembrane glycoprotein containing a single Link domain, responsible for hyaluronan binding. Bovine CD44 is expressed by a wide range of bovine cells, including peripheral T and B lymphocytes, monocytes, granulocytes, platelets and early erythroid cells.
    • Intended Use
    • Target Species
      Bovine
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Camelyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
      1%Bovine Serum Albumin
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.1 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised BALB/c mice were fused with cells of the X63.Ag8.853 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light.
      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      integral to membrane
      cell adhesion
      hyaluronic acid binding
      receptor activity
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD44 Antibody Formats

    Formats Clone Applications Sizes available
    CD44 Antibody : FITC IL-A118 F 0.1 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG1 Negative Control:FITCMCA928F100 TestsF
        MCA928F

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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