CD32 Antibody | CCG39

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CD32 Antibody | CCG39 gallery image 1

Staining of bovine peripheral blood lymphocytes with Mouse anti Bovine CD32 (MCA5919GA) followed by Goat anti Mouse IgG:FITC (STAR117F)

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CD32 Antibody | CCG39 gallery image 2

Staining of bovine peripheral blood lymphocytes with Mouse anti Bovine CD32:FITC (MCA5919F)

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  • CD32 Antibody | CCG39 thumbnail image 1
  • CD32 Antibody | CCG39 thumbnail image 2
  • Mouse anti Bovine CD32
  • Mouse anti Bovine CD32:FITC
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  • Product Type
    Monoclonal Antibody
  • Clone
    CCG39
  • Isotype
    IgG2a
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA5919GAC, Fdatasheet pdfdatasheet pdf0.1 mg
    MCA5919GA
    MCA5919FFdatasheet pdfdatasheet pdf0.1 mg
    MCA5919F
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Mouse anti Bovine CD32 antibody, clone CCG39, recognizes the bovine homologue of human CD32, one of a group of Fc receptors belonging to the immunoglobulin superfamily and involved in phagocytosis of opsonized microbes. Bovine CD32 is a single pass type 1 membrane protein of approximately 32kDa, expressed on the cell surface of most cells including B-lymphocytes, monocytes, neutrophils and afferent veiled lymph dendritic cells Chattha et al. 2010. It has been shown that expression of bovine CD32 is higher on macrophages than on neutrophils.

      CD32 can function in an inhibtory capacity to antibody production and is the low affinity Fc receptor for IgG (FcRII), binding to the Fc region of immunoglobulin gamma Chattha et al. 2009.

      Mouse anti Bovine CD32, clone CCG39 is one of a number of anti bovine CD32 reagents available from Bio-Rad, clone CCG39 is of interest in that it is specific to bovine CD32 and does not recognizes ovine CD32. For detection of ovine CD32, clone CCG36, which cross reacts with ovine CD32, may be used.
    • Intended Use
    • Target Species
      Bovine
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Sheepno
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - Liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
    • Immunogen
      Bovine FcγRII-transfected COS7 cells.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
      IgG concentration 0.1 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised BALB/c mice were fused with cells of the NS-1myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light.
      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch
      18 months from date of despatch
    • GO Terms
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/101/50
      Immunohistology - Frozen
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional CD32 Antibody Formats

    Formats Clone Applications Sizes available
    CD32 Antibody : FITC CCG39 F 0.1 mg
    CD32 Antibody : Purified CCG39 C, F 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
      STAR117A
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      STAR87A
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
      STAR117D488GA
      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF
      STAR117D549GA
      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      STAR117D649GA
      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
      STAR117D680GA
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      STAR117D800GA
      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      STAR8D800GA
      Human anti Mouse IgG2a:FITCHCA037F0.1 mgF
      HCA037F
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      STAR117F
      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
      STAR70
      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
      STAR120F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      STAR9B
      Human anti Mouse IgG2a:HRPHCA037P0.1 mgE
      HCA037P
      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      STAR117P
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      STAR77
      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
      STAR120P
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
      STAR13B
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      STAR87P
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      STAR12A
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
      STAR76

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG2a Negative ControlMCA929100 TestsF
        MCA929
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse IgG2a Negative Control:FITCMCA929F100 TestsF
        MCA929F

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse anti Bovine CD32:FITCMCA5918F0.1 mgF
          MCA5918F
          Mouse anti Bovine CD32MCA5918GA0.1 mgC, F
          MCA5918GA
          Mouse anti Bovine CD32:RPEMCA5918PE100 TestsF
          MCA5918PE
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse anti Bovine CD32:FITCMCA5918F0.1 mgF
          MCA5918F
          Mouse anti Bovine CD32MCA5918GA0.1 mgC, F
          MCA5918GA
          Mouse anti Bovine CD32:RPEMCA5918PE100 TestsF
          MCA5918PE

          Recommended Positive Controls

            Histology Controls

              Further Reading

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