CD3 Antibody | MM1A

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CD3 Antibody | MM1A gallery image 1

Flow cytometric staining of bovine peripheral blood lymphocytes.
A. Mouse IgG1 negative control (MCA928) followed by Goat anti Mouse IgG1:PE (STAR132PE).
B. Mouse anti Bovine CD3 antibody, clone MM1A (MCA6080) followed by Goat anti Mouse IgG1:PE secondary antibody (STAR132PE).
Both the Mouse IgG1 negative control and Mouse anti Bovine CD3 are plotted against bovine CD4 as recognized by FITC conjugated Mouse anti Bovine CD4 antibody, clone CC8 (MCA1653F).

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  • Mouse anti Bovine CD3
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  • Product Type
    Monoclonal Antibody
  • Clone
    MM1A
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA6080F, ICdatasheet pdfdatasheet pdf0.1 mg
    MCA6080
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Mouse anti Bovine CD3, clone MM1A, recognizes bovine CD3. CD3 plays an important role in T cell activation and in expression of intact TCR molecules. Clone MM1A detects bovine CD3 and reacts with bovine alpha beta and gamma delta TCR+ T cells and recognizes an epitope located on the epsilon chain of CD3 (N.D. MacHugh et al. 1998). Clone MM1A was also shown to react with the zebu bovid (Conrad et al. 2007).
    • Intended Use
    • Target Species
      Bovine
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Bisonyes
      Water Buffaloyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Concentrated tissue culture supernatant - liquid
    • Reconstitution
    • Preparation
      Concentrated tissue culture supernatant clarified by filtration through a 0.2 micrometer filter
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Monocyte enriched bovine mononuclear cells
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Serum free tissue culture medium containing proprietary protein free supplement
    • Storage
      Store at +4oC. DO NOT FREEZE.
      Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch
    • GO Terms
      transmembrane receptor activity
      cell surface receptor linked signaling pathway
      integral to membrane
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
      Immunocytochemistry

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD3 Antibody Formats

    Formats Clone Applications Sizes available
    CD3 Antibody : Con S/N MM1A F, IC 0.1 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG1:RPESTAR132PE0.1 mgF
      STAR132PE

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Antibody Characterization Reference

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