CD282 Antibody | 12538

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CD282 Antibody | 12538 gallery image 1

Bovine peripheral monocytes stained with Human anti Bovine CD282 Alexa Fluor®647 (HCA152A647) right hand image or HuCAL Fab-dHLX-MH Negative control (HCA052A647) left hand image. Mouse anti Bovine CD14:FITC (MCA1568F) was used as a dual stain in both cases

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CD282 Antibody | 12538 gallery image 2

Bovine peripheral monocytes stained with Human anti Bovine CD282 :FITC (HCA152F) right hand image or HuCAL Fab-dHLX-MH Negative control FITC (HCA052F) left hand image. Mouse anti Bovine CD14:FITC (MCA1568F) was used as a dual stain in both cases

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  • CD282 Antibody | 12538 thumbnail image 1
  • CD282 Antibody | 12538 thumbnail image 2
  • Human anti Bovine CD282:FITC
  • Human anti Bovine CD282
  • Human anti Bovine CD282:Alexa Fluor® 647
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    12538
  • Isotype
    HuCAL Fab bivalent
3 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    HCA152FFdatasheet pdfdatasheet pdf0.1 mg
    HCA152F
    HCA152Fdatasheet pdfdatasheet pdf0.1 mg
    HCA152
    HCA152A647Fdatasheet pdfdatasheet pdf100 Tests/1ml
    HCA152A647
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Human anti Bovine CD282 antibody, clone 12538 recognizes bovine TLR2, otherwise known as CD282. TLR2 is a single-pass type 1 membrane protein belong to the Toll-like receptor (TLR) family and is expressed primarily by peripheral blood monocytes.

      TLRs are expressed on the cell surface and the endocytic compartment and recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents. They also initiate cell signalling to induce production of cytokines necessary for the innate immunity and subsequent adaptive immunity.

      TLR2 is reported to respond to a diverse range of bacterial cell wall components, mediating the innate immune response in co-operation with Ly96 and TLR1.
    • Intended Use
    • Target Species
      Bovine
    • Product Form
      A bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is conjugated to fluorescein isothiocyanate (FITC) - liquid.
      A bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied liquid.
      A bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is conjugated to Alexa Fluor 647 - liquid.
    • Reconstitution
    • Preparation
      Metal chelate affinity chromatography
      Metal chelate affinity chromatography
      Metal chelate affinity chromatography
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      0.09% Sodium Azide (NaN3)
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
    • Immunogen
      Fc-fusion protein containing the sequence 21-588 from bovine TLR2.
    • Purity
    • Approx. Protein Concentrations
      Total protein concentration 0.1mg/ml
      Total protein concentration 0.5mg/ml
      Total protein concentration 0.05mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light.
      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light.
      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch.
      12 months from date of despatch.
      12 months from date of despatch.
    • GO Terms
      integral to membrane
      innate immune response
      transmembrane receptor activity
      inflammatory response
    • UniProt
    • Entrez Gene
    • Acknowledgements
      Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents.
      Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents.
      Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents.

      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
    • Licensed Use
      For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/101/50
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD282 Antibody Formats

    Formats Clone Applications Sizes available
    CD282 Antibody : Alexa Fluor® 647 12538 F 100 Tests/1ml
    CD282 Antibody : FITC 12538 F 0.1 mg
    CD282 Antibody : Purified 12538 F 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Mouse anti Histidine Tag:Alexa Fluor® 488MCA1396A488100 Tests/1mlF
      MCA1396A488
      Mouse anti c-Myc:Alexa Fluor® 488MCA2200A488100 Tests/1mlF *
      MCA2200A488
      Mouse anti Histidine Tag:Alexa Fluor® 647MCA1396A6471 mlF
      MCA1396A647
      Mouse anti c-Myc:Alexa Fluor® 647MCA2200A647100 Tests/1mlF *
      MCA2200A647
      Mouse anti c-Myc:BiotinMCA2200B0.1 mgC, E, P, WB*
      MCA2200B
      Mouse anti Histidine Tag:FITCMCA1396F0.1 mgF, IF
      MCA1396F
      Mouse anti c-Myc:FITCMCA2200F0.1 mgF *
      MCA2200F
      Mouse anti Penta Histidine Tag:HRPMCA5995P125 µlE, WB
      MCA5995P
      Mouse anti c-Myc:RPEMCA2200PE100 TestsWB
      MCA2200PE

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        HuCAL Fab-dHLX-MH Negative Control:FITCHCA052F0.1 mgC, E, F, P, WB
        HCA052F
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        HuCAL Fab-dHLX-MH Negative ControlHCA0520.1 mgC, E, F, P, WB
        HCA052
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        HuCAL Fab-dHLX-MH Negative Control:Alexa Fluor® 647HCA052A6471 mlE, F
        HCA052A647

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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