Flow cytometric staining of bovine peripheral blood lymphocytes. A. Mouse IgG2b negative control (MCA691) followed by Goat anti Mouse IgG2b:PE (STAR134PE). B. Mouse anti Bovine CD26 antibody, clone CACT114A (MCA6087) followed by Goat anti Mouse IgG2b:PE secondary antibody (STAR134PE). Both the Mouse IgG2b negative control and Mouse anti Bovine CD26 are plotted against bovine CD40 as recognized by FITC conjugated Mouse anti Bovine CD40 antibody, clone IL-A156 (MCA2431F).
Mouse anti Bovine CD26, clone CACT114A, recognizes bovine CD26 also known as DPP4 or dipeptidyl peptidase 4. Bovine CD26 is expressed in various cell types and participates in many biological activities such as the post-translational processing of chemokines (Proost et al. 2001) and neuropeptides (Lambeir et al. 2001), and in the regulation of degradation and inactivation of glucagon (Pospisilik et al. 2001). CD26 also functions as a costimulatory molecule on activated T cells and is highly expressed on CD8+ natural killer cells in calves vaccinated against Mycobacterium avium paratuberculosis (Park et al. 2015).
Concentrated tissue culture supernatant - liquid
Concentrated tissue culture supernatant clarified by filtration through a 0.2 micrometer filter
0.09% Sodium Azide (NaN3)
ConA activated bovine lymphocytes
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Reagents In The Kit
Preparing The Antibody
Serum free tissue culture medium containing proprietary protein free supplement
Store at +4oC. DO NOT FREEZE. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Use 10ul of the suggested working dilution to label 106 cells in 100ul