CD172a Antibody | DH59B

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CD172a Antibody | DH59B gallery image 1

Flow cytometric staining of bovine peripheral blood monocytes.
A. Mouse IgG1 negative control (MCA928) followed by Goat anti Mouse IgG1:PE (STAR132PE).
B. Mouse anti Bovine CD172a antibody, clone DH59B (MCA6079) followed by Goat anti Mouse IgG1:PE secondary antibody (STAR132PE).
Both the Mouse IgG1 negative control and Mouse anti Bovine CD172a are plotted against bovine CD11b as recognized by FITC conjugated Mouse anti Bovine CD11b antibody, clone CC126 (MCA1425F).

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  • CD172a Antibody | DH59B thumbnail image 1
  • Mouse anti Bovine CD172a
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  • Product Type
    Monoclonal Antibody
  • Clone
    DH59B
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA6079C, Fdatasheet pdfdatasheet pdf0.1 mg
    MCA6079
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Mouse anti Bovine CD172a, clone DH59B, recognizes bovine CD172a also known as SIRP alpha or MyD-1 antigen. CD172a is a transmembrane signal regulatory protein expressed primarily by macrophages, monocytes, dendritic cells, granulocytes, myeloid progenitors, hematopoietic stem cells, and neurons (Barclay et al. 2006). The extracellular region of SIRP family consists of three immunoglobulin superfamily (IgSF) domains; two IgC and one IgV domain (Barclay et al. 2006).

      The IgV domain of CD172a binds to CD47 (Hatherley et al. 2007).The binding domain of CD172a is analogous to that of immunoglobulins and T cell receptors and is involved in myeloid cell activation (Berg et al.2004). However, signaling via CD172a is mainly inhibitory to cell function and phagocytosis (Oldenborg et al. 2001). CD172a expressing cells are stimulated in Mycobacterium tuberculosis infection, influencing migration of dendritic cells and macrophages, phagocytosis, and granuloma formation (Waters et al. 2009).
    • Intended Use
    • Target Species
      Bovine
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Bisonyes
      Water Buffaloyes
      Sheepyes
      Horseyes
      Dogyes
      Catyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Concentrated tissue culture supernatant - liquid
    • Reconstitution
    • Preparation
      Concentrated tissue culture supernatant clarified by filtration through a 0.2 micrometer filter
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Cells from multiple species with the final screening of the fusion on cells from dog
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Serum free tissue culture medium containing proprietary protein free supplement
    • Storage
      Store at +4oC. DO NOT FREEZE.
      Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch
    • GO Terms
      integral to membrane
      SH3 domain binding
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
      Immunohistology - Frozen

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD172a Antibody Formats

    Formats Clone Applications Sizes available
    CD172a Antibody : Con S/N DH59B C, F 0.1 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)STAR117A0.5 mgE, WB
      STAR117A
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      STAR87A
      Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)STAR117D488GA0.1 mgF, IF
      STAR117D488GA
      Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)STAR117D549GA0.1 mgF, IF, WB
      STAR117D549GA
      Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)STAR117D649GA0.1 mgF, IF
      STAR117D649GA
      Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)STAR117D680GA0.1 mgF, WB
      STAR117D680GA
      Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)STAR117D800GA0.1 mgF, IF, WB
      STAR117D800GA
      Rabbit F(ab')2 anti Mouse IgG:Dylight®800STAR8D800GA0.1 mgF, IF, WB
      STAR8D800GA
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      STAR117F
      Goat anti Mouse IgG:FITC (Rat Adsorbed)STAR700.5 mgF
      STAR70
      Goat anti Mouse IgG (Fc):FITCSTAR120F1 mgC, F
      STAR120F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      STAR9B
      Human anti Mouse IgG1:HRPHCA036P0.1 mgE
      HCA036P
      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      STAR117P
      Goat anti Mouse IgG:HRP (Rat Adsorbed)STAR770.5 mgC, E, P
      STAR77
      Goat anti Mouse IgG (Fc):HRPSTAR120P1 mgE, WB
      STAR120P
      Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)STAR13B1 mgC, E, P, RE, WB
      STAR13B
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      STAR87P
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      STAR12A
      Goat anti Mouse IgG:RPE (Rat Adsorbed)STAR761 mlF
      STAR76

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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