CD11c Antibody | BAQ153A

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CD11c Antibody | BAQ153A gallery image 1

Flow cytometric staining of bovine peripheral blood monocytes.
A. Mouse IgM negative control (MCA692) followed by Goat anti Mouse IgM:PE (STAR86PE).
B. Mouse anti Bovine CD11c antibody, clone BAQ153A (MCA6084) followed by Goat anti Mouse IgM:PE secondary antibody (STAR86PE).
Both the Mouse IgM negative control and Mouse anti Bovine CD11c are plotted against bovine CD11b as recognized by FITC conjugated Mouse anti Bovine CD11b antibody, clone CC126 (MCA1425F).

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  • Mouse anti Bovine CD11c
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  • Product Type
    Monoclonal Antibody
  • Clone
    BAQ153A
  • Isotype
    IgM
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA6084F, ICdatasheet pdfdatasheet pdf0.1 mg
    MCA6084
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Mouse anti Bovine CD11c, clone BAQ153A, recognizes bovine CD11c also known as integrin alpha X. CD11c is a receptor for fibrinogen recognizing the G-P-R motif in fibrinogen. It is involved in the cell to cell interaction during inflammatory responses. In addition, it has an important function in adhesion and chemotaxis in monocytes, macrophages and dendritic cells (DCs).

      In cattle, CD11c+ DCs are a source of the anti-inflammatory cytokine IL-10 during infection by the highly pathogenic foot-and-mouth disease virus (FMDV). IL-10 may be responsible for the reduction in the antigen processing ability of DCs during FMDV infection (Sei et al. 2016). Clone BAQ153A reacts with bovine CD11c+ macrophages, DCs and some lymphocytes as shown by IHC (Palmer et al. 2011) and flow cytometry (Sei et al. 2016).
    • Intended Use
    • Target Species
      Bovine
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Goatyes
      Sheepyes
      Bisonyes
      Water Buffaloyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Concentrated tissue culture supernatant - liquid
    • Reconstitution
    • Preparation
      Concentrated tissue culture supernatant clarified by filtration through a 0.2 micrometer filter
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Cells from multiple species
    • Purity
    • Approx. Protein Concentrations
      Ig concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Serum free tissue culture medium containing proprietary protein free supplement
    • Storage
      Store at +4oC. DO NOT FREEZE.
      Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
      Functional Assays
      Immunocytochemistry

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD11c Antibody Formats

    Formats Clone Applications Sizes available
    CD11c Antibody : Con S/N BAQ153A F, IC 0.1 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgM:RPESTAR86PE0.5 mlF
      STAR86PE

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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