Carboxypeptidase A1 Antibody

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Carboxypeptidase A1 Antibody gallery image 1

Staining of formalin fixed, paraffin embedded human pancreas with Rabbit anti Bovine carboxypeptidase 1 (AHP2054)

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Immunoperoxidase staining of human pancreas with Rabbit anti Bovine carboxypeptidase A1 antibody (AHP2054) and HISTAR detection kit. Low power

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Carboxypeptidase A1 Antibody gallery image 3

Immunoperoxidase staining of human pancreas with Rabbit anti Bovine carboxypeptidase A1 antibody (AHP2054) and HISTAR detection kit. Med power

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Carboxypeptidase A1 Antibody gallery image 4

Immunoperoxidase staining of human pancreas with Rabbit anti Bovine carboxypeptidase A1 antibody (AHP2054) and HISTAR detection kit. High power

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Carboxypeptidase A1 Antibody gallery image 5

Published customer image:
Rabbit anti Bovine Carboxypeptidase A1 antibody used for the evaluation of carboxypepridase and pro-carboxypeptidase in mive tissue lysates by western blotting.
Image caption:
STC2 over-expression reduces severity of acinar cell damage during cerulein-induced pancreatic injury. (A) Analysis of serum amylase levels four hours into CIP treatment show that serum amylase levels rise approximately 3 fold in WT mice when compared to saline (•) whereas the increase in STC2Tg mice is significantly lower at 2-fold (¦; * p < 0.01). (B) Tissue edema analysis revealed significant increases for both WT and STC2Tg tissue 4 hours into CIP (black bars) when compared to saline controls (white bars; n = 4 animals). No difference between genotypes was observed. (C) Representative Western blot for procarboxypeptidase (47 kDa) and carboxypeptidase (CPA; 33 kDa) or trypsinogen four hours after initial saline (Sal.) or cerulein (CIP) injections showed decreased accumulation of activate CPA in STC2Tg mice four hours into CIP. Trypsinogen levels do not differ between genotypes. (D) Quantification by densitometry (graph) comparing the ratio of cleaved (active; 33 kDa) to total CPA revealed a significant increase in WT tissue and, to a significantly lower extent, in STC2Tg tissue (n = 4 animals, letters represent statistically different values; p < 0.05). IF analysis for β-catenin (E-H) or Cx32 (I-L) in wild type (WT) or STC2Tg (Tg) mice four hours after initial injection of saline (E, G, I, K) or cerulein (F, H, J, L) showing decreased accumulation only in WT tissue during CIP. (M) Representative Western blot analysis on pancreatic protein extracts 4 hours after initial saline or cerulein (CIP) injection confirm decreased β-catenin and Cx32 accumulation only in WT-CIP treated tissue (n = 4).

From: Fazio EN, Dimattia GE, Chadi SA, Kernohan KD, Pin CL. Stanniocalcin 2 altersPERK signalling and reduces cellular injury during cerulein induced pancreatitis in mice.
BMC Cell Biol. 2011 May 5;12:17.

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  • Rabbit anti Bovine Carboxypeptidase A1
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP2054E, IF, P, WBdatasheet pdfdatasheet pdf50 µg
    AHP2054
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rabbit anti Bovine Carboxypeptidase A1 antibody recognizes bovine Carboxypeptidase A1, a 309 amino acid monomeric pancreatic extopeptidase derived by cleavage of the propeptide. Carboxypeptidase A1 belongs to the peptidase M14 family (UniProt: P00370).
    • Intended Use
    • Target Species
      Bovine
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Humanyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Ig fraction - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antiserum to bovine carboxypeptidase A1 was raised by repeated immunisation of rabbits with highly purified antigen. IgG fraction was prepared from whole serum by ammonium sulphate precipitation.
    • Preservative Stabilisers
      0.01% Sodium Azide (NaN3)
    • Immunogen
      Carboxypeptidase A1 from bovine pancreas.
    • Purity
    • Approx. Protein Concentrations
      Total protein concentration 1.0 mg/ml.
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      zinc ion binding
      proteolysis
      extracellular space
      metallocarboxypeptidase activity
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Immunofluorescence
      Immunohistology - Paraffin5ug/ml
      Western Blotting

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
      This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
    • Histology Positive Control Tissue
      Bovine pancreas
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Carboxypeptidase A1 Antibody Formats

    Formats Applications Sizes available
    Carboxypeptidase A1 Antibody : Purified E, IF, P, WB 50 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      STAR54

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          BUF025C
          TidyBlot Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          STAR209P
          Histar Detection SystemSTAR3000B150 TestsC, P
          STAR3000B
          Histar Detection SystemSTAR3000A50 TestsC, P
          STAR3000A
          Histar Detection SystemSTAR3000C500 TestsC, P
          STAR3000C
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP
          BUF025A

          Recommended Positive Controls

            Histology Controls

              Bovine pancreas

              References

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