DC059 is suitable for use as a negative control for the measurement of non-specific binding of rat monoclonals of isotype IgG2b and IgG2a to mouse tissues, in dual labelling technique using FITC and R-Phycoerythrin fluorochromes.
- Target Species
- Negative Control
- Product Form
- Dual Colour combination consisting of FITC conjugated and RPE conjugated monoclonal antibodies mixed in optimal ratio - lyophilized
- Reconstitute with 1.0 ml distilled water
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
|1%||Bovine Serum Albumin|
- Antibody Isotypes
- FITC reagent: IgG2b (RAT)
RPE reagent: IgG2a (RAT)
- Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
- 12 months from date of reconstitution
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Rat IgG2b/IgG2a Negative Control antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
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