Rat IgG2b/IgG2a Negative Control antibody

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Rat IgG2b:FITC/Rat IgG2a:RPE Negative Control

Product Type
Negative/Isotype Control
Isotype
IgG2a
IgG2b
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
DC059 F 100 Tests
DC059 is suitable for use as a negative control for the measurement of non-specific binding of rat monoclonals of isotype IgG2b and IgG2a to mouse tissues, in dual labelling technique using FITC and R-Phycoerythrin fluorochromes.

Product Details

Target Species
Negative Control
Product Form
Dual Colour combination consisting of FITC conjugated and RPE conjugated monoclonal antibodies mixed in optimal ratio - lyophilized
Reconstitution
Reconstitute with 1.0 ml distilled water
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Antibody Isotypes
FITC reagent: IgG2b (RAT)
RPE reagent: IgG2a (RAT)

Storage Information

Storage
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Shelf Life
12 months from date of reconstitution

More Information

Regulatory
For research purposes only

Applications of Rat IgG2b/IgG2a Negative Control antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul