Rat IgG2a/IgG2a Negative Control antibody

Rat IgG2a:FITC/Rat IgG2a:RPE Negative Control

Product Type
Negative/Isotype Control
Isotype
Cocktail
Specificity
Rat IgG2a/IgG2a Negative Control

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DC036
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Rat IgG2a FITC / Rat IgG2a RPE negative control is suitable for use as a negative control for the measurement of non-specific binding of rat monoclonals of isotype IgG2a to mouse tissues in a dual labelling technique using FITC and R-Phycoerythrin fluorochromes.

Target Species
Negative Control
Product Form
Dual Colour combination consisting of FITC conjugated and RPE conjugated monoclonal antibodies mixed in optimal ratio - lyophilized
Reconstitution
Reconstitute with 1 ml distilled water
Preparation
Antibody purified from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Antibody Isotypes
FITC reagent: IgG2a (RAT)
RPE reagent: IgG2a (RAT)
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
RPE 488nm laser 496 578
RPE 561nm laser 546 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

RRID
AB_324566
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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