Rat IgG2a/IgG2a Negative Control antibody
DC036 is suitable for use as a negative control for the measurement of non-specific binding of rat monoclonals of isotype IgG2a to mouse tissues in a dual labelling technique using FITC and R-Phycoerythrin fluorochromes.
- Target Species
- Negative Control
- Product Form
- Dual Colour combination consisting of FITC conjugated and RPE conjugated monoclonal antibodies mixed in optimal ratio - lyophilised.
- Reconstitute with 1 ml distilled water
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose
- Antibody Isotypes
- FITC reagent: IgG2a (RAT)
RPE reagent: IgG2a (RAT)
- Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
- Shelf Life
- 12 months from date of reconstitution.
- For research purposes only
Applications of Rat IgG2a/IgG2a Negative Control antibody
|Application Name||Verified||Min Dilution||Max Dilution|
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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