Rat IgG2a/IgG1 Negative Control antibody

Rat IgG2a:FITC/Rat IgG1:RPE Negative Control

Product Type
Negative/Isotype Control
Isotype
IgG1
IgG2a
Specificity
Rat IgG2a/IgG1 Negative Control

Product Code Applications Pack Size List Price Your Price Qty
DC057
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DC057 is suitable for use as a negative control for the measurement of non-specific binding of rat monoclonals of isotype IgG2a and IgG1 to human tissues in a dual labelling technique using FITC and R-Phycoerythrin fluorochromes.

Suitable for use as negative control for human, pig, dog and mouse.

Target Species
Negative Control
Product Form
Dual Colour combination consisting of FITC conjugated and RPE conjugated monoclonal antibodies mixed in optimal ratio - lyophilized
Reconstitution
Reconstitute with 0.5 ml distilled water
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Antibody Isotypes
FITC reagent: IgG2a (RAT)
RPE reagent: IgG1 (RAT)
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
RPE 488nm laser 496 578
RPE 561nm laser 546 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul to label 106 cells or 100ul whole blood

Description Product Code Applications Pack Size List Price Your Price Quantity
Rat IgG2a:FITC/Rat IgG2a:RPE Negative Control DC036 F 100 Tests loader
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Description Rat IgG2a:FITC/Rat IgG2a:RPE Negative Control
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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