Multiple IgG1/IgG2a Negative Control antibody
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Mouse IgG1:FITC/Rat IgG2a:RPE Negative Control
- Product Type
- Negative/Isotype Control
- Isotype
- IgG1
- IgG2a
- Specificity
- Multiple IgG1/IgG2a Negative Control
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| DC058 is suitable for use as a negative control for the measurement of non-specific binding of mouse monoclonals of isotype IgG1 and rat monoclonals of isotype IgG2a to human tissues in a dual labelling technique using FITC and R-Phycoerythrin fluorochromes. DC058 recognises a rat cell surface antigen and therefore cannot be used as a negative control in this species. |
- Target Species
- Negative Control
- Product Form
- Dual Colour combination consisting of FITC conjugated and RPE conjugated monoclonal antibodies mixed in optimal ratio - lyophilized
- Reconstitution
- Reconstitute with 0.5 ml distilled water
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Antibody Isotypes
- FITC reagent: IgG1 (MOUSE)
RPE reagent: IgG2a (RAT) - Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) FITC 490 525 RPE 488nm laser 496 578 RPE 561nm laser 546 578 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul to label 106 cells or 100ul whole blood
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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