Multiple IgG1/IgG2a/IgG1 Negative Control antibody

Mse IgG1:FITC/Rat IgG2a:RPE/Rat IgG1:RPE-Alexa Fluor® 647-ve Control

Product Type
Negative/Isotype Control
Isotype
Cocktail
Specificity
Multiple IgG1/IgG2a/IgG1 Negative Control

Product Code Applications Pack Size List Price Your Price Qty
TC024
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F * 100 Tests/1ml loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Multiple IgG1/IgG2a/IgG1 negative control is a suitable isotype control for the measurement of non-specific binding of mouse IgG1, rat IgG2a and rat IgG1 monoclonal antibodies, conjugated to FITC, RPE and RPE-Alexa Fluor ® 647 respectively, in three colour flow cytometry experiments.

This product is appropriate for use in experiments targeting human, canine and porcine cells.

Target Species
Negative Control
Product Form
Triple Colour combination consisting of FITC, RPE and RPE- Alexa Fluor® 647 conjugated monoclonal antibodies mixed in optimal ratio - lyophilized
Reconstitution
Reconstitute with 1.0 ml distilled water
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Antibody Isotypes
FITC reagent: IgG1 (MOUSE)
RPE reagent: IgG2a (RAT)
RPE-A647 reagent: IgG1 (RAT)
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
RPE 488nm laser 496 578
RPE 561nm laser 546 578
Alexa Fluor®647 650 665
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com

Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1
  1. 1It is recomended that the user dilutes the antibody for use in their own system to a concentration equivalent to their test reagent.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul to label 106 cells or 100ul whole blood