Mouse IgM/IgG1/IgG1 Negative Control antibody

Mouse IgM:FITC/IgG1:RPE/IgG1:Alexa Fluor® 647 -ve Control

Product Type
Negative/Isotype Control
Mouse IgM/IgG1/IgG1 Negative Control

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Mouse IgM FITC / Mouse IgG1 RPE / Mouse IgG1 Alexa Fluor®647 negative control is suitable for use as a negative control for the measurement of non-specific binding of mouse monoclonal antibodies, of isotype IgM and IgG1, to rat tissues in a triple labelling technique using FITC, RPE and Alexa Fluor® 647 fluorochromes.

Target Species
Negative Control
Product Form
Triple Colour combination consisting of FITC, RPE and Alexa Fluor® 647 conjugated monoclonal antibodies mixed in optimal ratio - lyophilized
Reconstitute with 0.5 ml distilled water
Antibody purified from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
1%Bovine Serum Albumin
Antibody Isotypes
FITC reagent Mouse IgM
RPE reagent Mouse IgG1
A647 reagent Mouse IgG1
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
RPE 488nm laser 496 578
RPE 561nm laser 546 578
Alexa Fluor®647 650 665
For research purposes only
12 months from date of despatch
The Alexa Fluor® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays, and are covered by pending and issued patents.

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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