Mouse IgM/IgG1/IgG1 Negative Control antibody
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Mouse IgM:FITC/IgG1:RPE/IgG1:Alexa Fluor® 647 -ve Control
- Product Type
- Negative/Isotype Control
- Isotype
- Cocktail
- Specificity
- Mouse IgM/IgG1/IgG1 Negative Control
Antibody Quality and Performance Guarantee
| Mouse IgM FITC / Mouse IgG1 RPE / Mouse IgG1 Alexa Fluor®647 negative control is suitable for use as a negative control for the measurement of non-specific binding of mouse monoclonal antibodies, of isotype IgM and IgG1, to rat tissues in a triple labelling technique using FITC, RPE and Alexa Fluor® 647 fluorochromes. |
- Target Species
- Negative Control
- Product Form
- Triple Colour combination consisting of FITC, RPE and Alexa Fluor® 647 conjugated monoclonal antibodies mixed in optimal ratio - lyophilized
- Reconstitution
- Reconstitute with 0.5 ml distilled water
- Preparation
- Antibody purified from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide (NaN3) 1% Bovine Serum Albumin 5% Sucrose - Antibody Isotypes
- FITC reagent Mouse IgM
RPE reagent Mouse IgG1
A647 reagent Mouse IgG1 - Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) FITC 490 525 RPE 488nm laser 496 578 RPE 561nm laser 546 578 Alexa Fluor®647 650 665 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- The Alexa Fluor® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays, and are covered by pending and issued patents.
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
- RRID
- AB_1222744
View more products with MOUSE IG specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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