Mouse IgG2a/IgG2a Negative Control antibody
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Mouse IgG2a:FITC/Mouse IgG2a:RPE Negative Control
- Product Type
- Negative/Isotype Control
- Isotype
- Cocktail
- Specificity
- Mouse IgG2a/IgG2a Negative Control
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| Mouse IgG2a FITC / Mouse IgG2a RPE negative control is suitable for use as a negative control for the measurement of non-specific binding of mouse monoclonals of isotype IgG2a to human tissues in a dual labelling technique using FITC and R-Phycoerythrin fluorochromes. |
- Target Species
- Negative Control
- Product Form
- Dual colour combination consisting of FITC conjugated and RPE conjugated monoclonal antibodies mixed in optimal ratio - lyophilized
- Reconstitution
- Reconstitute with 0.5ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. - Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose - Antibody Isotypes
- FITC reagent: IgG2a (MOUSE)
RPE reagent: IgG2a (MOUSE) - Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) FITC 490 525 RPE 488nm laser 496 578 RPE 561nm laser 546 578 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Store at +4oC.
DO NOT FREEZE
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
DO NOT FREEZE
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of reconstituted product to label 106 cells in 100ul.
- RRID
- AB_322253
View more products with MOUSE IG specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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