Mouse IgG1 Negative Control antibody

Mouse IgG1 Negative Control:RPE-Alexa Fluor® 750

Product Type
Negative/Isotype Control
Mouse IgG1 Negative Control

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Mouse IgG1 Negative Control antibody is suitable for use as a negative control to assess non-specific binding of mouse IgG1 antibodies to target cells. Mouse IgG1 Negative Control antibody has been tested and found to be negative on the following rat cell types, peripheral blood leucocytes, thymocytes, splenocytes and macrophages.

Clone F8-11-13 recognises the human CD45RA antigen, and therefore human leucocytes may be used as a positive control for this product. NOT SUITABLE FOR USE AS A NEGATIVE CONTROL ON HUMAN TISSUES

Target Species
Negative Control
Product Form
Purified IgG conjugated to R. Phycoerythrin Alexa Fluor®750 - lyophilized
Reconstitute with 1.0 ml distilled water
Purified IgG prepared by ion exchange chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Human T lymphocytes.
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE-Alexa Fluor®750 488nm laser 496 779
RPE-Alexa Fluor®750 561nm laser 546 779
For research purposes only
6 months from date of despatch
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or

Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.


This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry *
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. * It is recommended that the user dilutes the antibody for use in their own system to a concentration equivalent to their test antibody.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul

References for Mouse IgG1 Negative Control antibody

  1. Weiss, D.J. et al. (2008) Bovine monocyte TLR2 receptors differentially regulate the intracellular fate of Mycobacterium avium subsp. paratuberculosis and Mycobacterium avium subsp. avium.
    J Leukoc Biol. 83 (1): 48-55.
  2. Chen, W. et al. (2009) Expression of toll-like receptor 4 in uvea-resident tissue macrophages during endotoxin-induced uveitis.
    Mol Vis. 15: 619-28.
  3. Safeukui I et al. (2015) Malaria induces anemia through CD8+ T cell-dependent parasite clearance and erythrocyte removal in the spleen.
    MBio. 6 (1) pii: e02493-14.
  4. Aricha, R. et al. (2016) Suppression of experimental autoimmune myasthenia gravis by autologous T regulatory cells.
    J Autoimmun. 67: 57-64.
  5. Wattegedera, S.R. et al. (2017) Enhancing the toolbox to study IL-17A in cattle and sheep.
    Vet Res. 48 (1): 20.
  6. Stangl, H. et al. (2020) MHC/class-II-positive cells inhibit corticosterone of adrenal gland cells in experimental arthritis: a role for IL-1β, IL-18, and the inflammasome.
    Sci Rep. 10 (1): 17071.

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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