HuCAL Fab-dHLX-MS Negative Control antibody | AbD22854

100% Secure

HuCAL Fab-dHLX-MS Negative Control

Product Type
Negative/Isotype Control
Clone
AbD22854
Isotype
HuCAL Fab bivalent
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
HCA048 C E P WB 0.1 mg
HuCAL Fab-dHLX-MS negative control, clone AbD22854 is a recombinant antibody with specificity for green fluorescent protein (GFP). It has no known reactivity with mammalian proteins or other antigens. It is therefore recommended as a negative control reagent when using other HuCAL antibodies of the same format.

It is recommended that this reagent is used at the same concentration as the test reagent.

Product Details

Target Species
Negative Control
Product Form
A lyophilized, bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library. Expressed in E. coli and purified using purified using NiNTA affinity chromatography. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with myc-tag (EQKLISEEDL) and a Strep-tag at the C-terminus of the antibody heavy chain.
Reconstitution
Reconstitute with 1.0 ml distilled water
Preparation
Affinity chromatography on a Strep-tag affinity column
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
Green fluorescent protein.
Approx. Protein Concentrations
Antibody concentration 0.1 mg/ml following reconstitution

Storage Information

Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Shelf Life
6 months from date of reconstitution

More Information

Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
Licensed Use
For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
Regulatory
For research purposes only

Applications of HuCAL Fab-dHLX-MS Negative Control antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunohistology - Frozen
Immunohistology - Paraffin
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Human IgG F(ab')2:HRP 0500-0099 0.5 ml E WB
Mouse anti c-Myc:HRP MCA2200P 0.1 mg C E P WB*
Mouse anti Strep-Tag Classic:HRP MCA2489P 75 µl E WB