HuCAL Fab-A-V5Sx2 Negative Control antibody | AbD11074
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HuCAL Fab-A-V5Sx2 negative control, clone AbD11074 is a recombinant Fab antibody fragment in the format Fab-A-V5Sx2 with specificity for green fluorescent protein (GFP). It has no known reactivity with mammalian proteins or other antigens. It has been tested by ELISA for specificity to GFP, but has not been tested in other applications. It is suggested as a negative control reagent when using other HuCAL antibodies of the same Fab format.
It is recommended that this reagent is used at the same concentration as the test reagent.
It is recommended that this reagent is used at the same concentration as the test reagent.
Product Details
- Target Species
- Negative Control
- Product Form
- A bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is bivalent by dimerization of the bacterial alkaline phosphatase fusion protein. The antibody is tagged with a V5 tag and a double extended Strep-tag (SAWSHPQFEKGGGSGGGSGGSAWSHPQFEK). This antibody is supplied lyophilized.
- Reconstitution
- Reconstitute with 1.0 ml distilled water
- Preparation
- StrepTactin affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Immunogen
- Green fluorescent protein.
- Approx. Protein Concentrations
- Antibody concentration 0.1 mg/ml following reconstitution
Storage Information
- Storage
- Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of reconstitution
More Information
- Acknowledgements
- Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
- Licensed Use
- For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
- Regulatory
- For research purposes only
Applications of HuCAL Fab-A-V5Sx2 Negative Control antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse anti Strep-Tag Classic:HRP | MCA2489P | E WB | 75 µl |
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| Rat anti DYKDDDDK Tag | MCA4764 | E F IF IP P WB | 0.2 mg |
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| Rat anti DYKDDDDK Tag:Alexa Fluor®647 | MCA4764A647 | F | 1 ml |
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| Rat anti DYKDDDDK Tag:FITC | MCA4764F | F IF | 0.1 mg |
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| Rat anti DYKDDDDK Tag:HRP | MCA4764P | E P WB | 0.1 mg |
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| Rat anti DYKDDDDK Tag:RPE | MCA4764PE | F | 100 Tests |
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