HuCAL Fab-A-V5H Negative Control antibody | AbD23509

100% Secure

HuCAL Fab-A-V5H Negative Control

Product Type
Negative/Isotype Control
Clone
AbD23509
Isotype
HuCAL Fab bivalent
Product Code Applications Pack Size List Price Quantity
HCA258
Datasheet
E
MSDS
0.1 mg loader

HuCAL Fab-A-V5H negative control, clone AbD23509 is a recombinant Fab antibody fragment in the format Fab-A-V5H with specificity for green fluorescent protein (GFP). It has no known reactivity with mammalian proteins or other antigens. It has been tested by ELISA for specificity to GFP, but has not been tested in other applications. It is suggested as a negative control reagent when using other HuCAL antibodies of the same Fab format.

It is recommended that this reagent is used at the same concentration as the test reagent.

Product Details

Target Species
Negative Control
Product Form
A bivalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in E. coli. This Fab fragment is bivalent by dimerization of the bacterial alkaline phosphatase fusion protein. The antibody is tagged with a V5 tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Green Fluorescent Protein
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
Guarantee
12 months from date of despatch.

More Information

Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
His-tag is a registered trademark of EMD Biosciences.
Licensed Use
For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
Regulatory
For research purposes only

Applications of HuCAL Fab-A-V5H Negative Control antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Mouse anti V5-Tag:Alk. Phos. MCA1360A E WB 0.1 mg
Mouse anti V5-Tag:Biotin MCA1360B C E WB 0.1 mg loader
Mouse anti V5-Tag MCA1360GA C E F IF IP R WB 0.1 mg loader
Mouse anti V5-Tag:HRP MCA1360P E WB 0.1 mg loader
Mouse anti Histidine Tag:Alk.Phos. MCA1396A E WB 0.1 mg
Mouse anti Histidine Tag:Biotin MCA1396B E WB 0.1 mg loader
Mouse anti Histidine Tag MCA1396GA E IP WB 0.1 mg loader
Mouse anti Penta Histidine Tag:HRP MCA5995P E WB 125 µl loader
Op-in to our ecommunications and set your preferences to start receiving resources, protocols, product updates, special offers, and more.