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Tropomodulin 1 Antibody | TRM-17 (Mab17)
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Enlarge- Mouse anti Tropomodulin 1 (N-Terminal)
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Product TypeMonoclonal Antibody
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CloneTRM-17 (Mab17)
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IsotypeIgG1
1 Formats Available
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
Summary
Secondary Antibodies
Negative Isotype Controls
Useful Reagents
Positive Controls
Histology Controls
More Images
References
Reviews
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- Mouse anti Tropomodulin 1 (N-Terminal) antibody, clone TRM-17 recognizes chicken tropomodulin 1, otherwise known as erythrocyte tropomodulin or E-Tmod, an evolutionary very highly conserved cytoskeletal protein .
Tropomodulin 1 is a sarcomeric protein highly expressed by erythrocytes, heart and skeletal muscle, which binds to and caps pointed ends of actin filaments to prevent thin filament elongation. The binding of the N-terminal region of Tmod1 with the actin-binding protein tropomyosin blocks the elongation and depolymerization of actin filaments, contributing to the geometry of the membrane skeleton.
Mouse anti Tropomodulin 1 (N-Terminal) antibody, clone TRM-17 recognizes an epitope between amino acid residues 6-57 within the N-terminal (NT) region of tropomodulin 1 and inhibits the binding of Tmod1 to tropomyosin.
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Intended Use
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Target SpeciesChicken
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Species Cross-Reactivity
N.B. Antibody reactivity and working conditions may vary between species.Target Species Cross Reactivity Birds 
Mammals Fish Expected from Sequence Insect Expected from Sequence Reptile Expected from Sequence -
Product FormPurified IgG - liquid
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Reconstitution
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PreparationPurified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
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Preservative Stabilisers0.05% Sodium Azide (NaN3)
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ImmunogenRecombinant chicken tropomodulin 1
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Purity
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Approx. Protein ConcentrationsIgG concentration 1.0 mg/ml
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Reagents In The Kit
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Preparing The Antibody
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Test Principle
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Buffer SolutionPhosphate buffered saline
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StorageStore at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. -
Shelf Life18 months from date of despatch.
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Acknowledgements
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RegulatoryFor research purposes only
- This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Yes No Min Dilution Max Dilution ELISA Immunofluorescence Western Blotting Functional Assays(1) (1)Removal of sodium azide is recommended prior to use in functional assays. Bio-Rad recommend EQU003 for this purpose.- Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended Protocol
- ELISA
- Immunohistology
- Histology Positive Control Tissue
- Immunofluorescence
- Western BlottingMCA4760 detects a band of approximately 40kDa in chick heart lysates.
- Instructions For Use
Additional Tropomodulin 1 Antibody Formats
| Formats | Clone | Applications | Sizes available |
|---|---|---|---|
| Tropomodulin 1 Antibody : Purified | TRM-17 (Mab17) | E, FN*, IF, WB | 0.25 mg |
- Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)
Recommended Secondary Antibody
Recommended Negative Isotype Control
Useful Reagents
Recommended Positive Controls
Histology Controls
References
1. Mudry, R.E. et al. (2003) The interaction of tropomodulin with tropomyosin stabilizes thin filaments in cardiac myocytes.J. Cell. Biol. 162: 1057-1068.
2. Almenar-Queralt, A. et al. (1999) Identification of a novel tropomodulin isoform, skeletal tropomodulin, that caps actin filament pointed ends in fast skeletal muscleJ Biol Chem 274: 28466-75
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Formerly known as AbD Serotec, a global supplier and manufacturer of antibodies, kits, and reagents.
Copyright © 2017 Bio-Rad Laboratories, Inc. All rights reserved
Copyright © 2017 Bio-Rad Laboratories, Inc. All rights reserved
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