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Tropomodulin 1 Antibody | TRM-17 (Mab17)

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  • Mouse anti Tropomodulin 1 (N-Terminal)

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  • Product Type
    Monoclonal Antibody
  • Clone
    TRM-17 (Mab17)
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA4760E, FN*, IF, WBdatasheet pdfdatasheet pdf0.25 mg
    MCA4760
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Tropomodulin 1 (N-Terminal) antibody, clone TRM-17 recognizes chicken tropomodulin 1, otherwise known as erythrocyte tropomodulin or E-Tmod, an evolutionary very highly conserved cytoskeletal protein .

      Tropomodulin 1 is a sarcomeric protein highly expressed by erythrocytes, heart and skeletal muscle, which binds to and caps pointed ends of actin filaments to prevent thin filament elongation. The binding of the N-terminal region of Tmod1 with the actin-binding protein tropomyosin blocks the elongation and depolymerization of actin filaments, contributing to the geometry of the membrane skeleton.

      Mouse anti Tropomodulin 1 (N-Terminal) antibody, clone TRM-17 recognizes an epitope between amino acid residues 6-57 within the N-terminal (NT) region of tropomodulin 1 and inhibits the binding of Tmod1 to tropomyosin.
    • Intended Use
    • Target Species
      Chicken
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Birdsyes
      Mammalsyes
      FishExpected from Sequence
      InsectExpected from Sequence
      ReptileExpected from Sequence
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
    • Preservative Stabilisers
      0.05% Sodium Azide (NaN3)
    • Immunogen
      Recombinant chicken tropomodulin 1
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Immunofluorescence
      Western Blotting
      Functional Assays(1)
      (1)
      Removal of sodium azide is recommended prior to use in functional assays. Bio-Rad recommend EQU003 for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      MCA4760 detects a band of approximately 40kDa in chick heart lysates.
    • Instructions For Use

    Additional Tropomodulin 1 Antibody Formats

    Formats Clone Applications Sizes available
    Tropomodulin 1 Antibody : Purified TRM-17 (Mab17) E, FN*, IF, WB 0.25 mg
    • Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

              1. Mudry, R.E. et al. (2003) The interaction of tropomodulin with tropomyosin stabilizes thin filaments in cardiac myocytes.
              J Cell Biol. 162 (6): 1057-68.
              2. Almenar-Queralt, A. et al. (1999) Identification of a novel tropomodulin isoform, skeletal tropomodulin, that caps actin filament pointed ends in fast skeletal muscle
              J Biol Chem 274: 28466-75

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