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Tropomodulin 1 Antibody | TRM-17 (Mab17)

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  • Mouse anti Tropomodulin 1 (N-Terminal)

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  • Product Type
    Monoclonal Antibody
  • Clone
    TRM-17 (Mab17)
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA4760E, FN*, IF, WBdatasheet pdfdatasheet pdf0.25 mg
    MCA4760
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Mouse anti Tropomodulin 1 (N-Terminal) antibody, clone TRM-17 recognizes chicken tropomodulin 1, otherwise known as erythrocyte tropomodulin or E-Tmod, an evolutionary very highly conserved cytoskeletal protein .

      Tropomodulin 1 is a sarcomeric protein highly expressed by erythrocytes, heart and skeletal muscle, which binds to and caps pointed ends of actin filaments to prevent thin filament elongation. The binding of the N-terminal region of Tmod1 with the actin-binding protein tropomyosin blocks the elongation and depolymerization of actin filaments, contributing to the geometry of the membrane skeleton.

      Mouse anti Tropomodulin 1 (N-Terminal) antibody, clone TRM-17 recognizes an epitope between amino acid residues 6-57 within the N-terminal (NT) region of tropomodulin 1 and inhibits the binding of Tmod1 to tropomyosin.
    • Intended Use
    • Target Species
      Chicken
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Birdsyes
      Mammalsyes
      FishExpected from Sequence
      InsectExpected from Sequence
      ReptileExpected from Sequence
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
    • Preservative Stabilisers
      0.05% Sodium Azide (NaN3)
    • Immunogen
      Recombinant chicken tropomodulin 1
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Immunofluorescence
      Western Blotting
      Functional Assays(1)
      (1)
      Removal of sodium azide is recommended prior to use in functional assays. Bio-Rad recommend EQU003 for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      MCA4760 detects a band of approximately 40kDa in chick heart lysates.
    • Instructions For Use

    Additional Tropomodulin 1 Antibody Formats

    Formats Clone Applications Sizes available
    Tropomodulin 1 Antibody : Purified TRM-17 (Mab17) E, FN*, IF, WB 0.25 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

              1. Mudry, R.E. et al. (2003) The interaction of tropomodulin with tropomyosin stabilizes thin filaments in cardiac myocytes.
              J. Cell. Biol. 162: 1057-1068.
              2. Almenar-Queralt, A. et al. (1999) Identification of a novel tropomodulin isoform, skeletal tropomodulin, that caps actin filament pointed ends in fast skeletal muscle
              J Biol Chem 274: 28466-75

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