Spectrin Antibody | AA6 (7A3)

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  • Mouse anti Chicken Spectrin (Non-Erythroid)
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    AA6 (7A3)
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    8365-1256CH, IF, IP, WBdatasheet pdfdatasheet pdf50 µg
    8365-1256
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Chicken spectrin, clone AA6, recognizes spectrin alpha chain, non-erythrocytic 1, also known as Alpha-II spectrin and Fodrin alpha chain, encoded by the SPTAN1 gene. Mouse anti chicken spectrin recognizes both chicken spectrin and the alpha chain of non-erythroid spectrin in mammalian cells.

      Clone AA6 reacts with the 240 kDa full length spectrin alpha, the ~150 kDa, 145kDa characteristic breakdown products of calpain and caspase-3 (Nath et al. 1996) and the ~120kDaproduct, a result of caspase-3 activation (Nath et al. 1998).
    • Intended Use
    • Target Species
      Chicken
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Rabbityes
      Ratyes
      Guinea Pigyes
      Humanyes
      Mouseyes
      Pigyes
      MammalsExpected from Sequence
      Humanyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
    • Immunogen
      Chicken blood cell membrane purified by hypotonic lysis and mechanical enucleation.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.1 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline.
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted.
      Avoid repeated freezing and thawing as this may denature the antibody.
      Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Chromatin Immunoprecipitation
      Immunofluorescence1/2001/400
      Immunohistology - Paraffin
      Immunoprecipitation
      Western Blotting1/5001/2000

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      Clone AA6 recognizes the 150kDa and 120kDa spectrin cleavage products in staurosporine-treated HeLa lysates.
    • Instructions For Use

    Additional Spectrin Antibody Formats

    Formats Clone Applications Sizes available
    Spectrin Antibody : Purified AA6 (7A3) CH, IF, IP, WB 50 µg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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