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Mouse anti Ovalbumin antibody, clone 2C6 used to generate a standard curve for murine IgE in a sandwich ELISA detected using biotin conjugated ovalbumin and streptavidin.
Analysis of OVA-specific IgE. The levels of IgE in sera obtained before, during, and after ASIT were detected and quantified by ELISA. The results are presented as mean IgE concentration (mean±SE, n = 8 for each). AD without ASIT: OVA-sensitized untreated mice; ASIT with OVA: OVA-sensitized mice treated by ASIT with OVA; ASIT with sOVA: OVA-sensitized mice treated by ASIT with sOVA; placebo: PBS-sensitized mice. (*, p<0.05).
From: Shershakova N, Bashkatova E, Babakhin A, Andreev S, Nikonova A, Shilovsky I, et al. (2015) Allergen-Specific Immunotherapy with Monomeric Allergoid in a Mouse Model of Atopic Dermatitis. PLoS ONE 10(8): e0135070.