Ovalbumin Antibody | 2C6

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Ovalbumin Antibody | 2C6 gallery image 1

Published customer image:
Mouse anti Ovalbumin antibody, clone 2C6 used to generate a standard curve for murine IgE in a sandwich ELISA detected using biotin conjugated ovalbumin and streptavidin.
Image caption:
Analysis of OVA-specific IgE. The levels of IgE in sera obtained before, during, and after ASIT were detected and quantified by ELISA. The results are presented as mean IgE concentration (mean±SE, n = 8 for each). AD without ASIT: OVA-sensitized untreated mice; ASIT with OVA: OVA-sensitized mice treated by ASIT with OVA; ASIT with sOVA: OVA-sensitized mice treated by ASIT with sOVA; placebo: PBS-sensitized mice. (*, p<0.05).

From: Shershakova N, Bashkatova E, Babakhin A, Andreev S, Nikonova A, Shilovsky I, et al. (2015) Allergen-Specific Immunotherapy with Monomeric Allergoid in a Mouse Model of Atopic Dermatitis. PLoS ONE 10(8): e0135070.

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  • Mouse anti Ovalbumin
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  • Product Type
    Monoclonal Antibody
  • Clone
    2C6
  • Isotype
    IgE
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2259Edatasheet pdfdatasheet pdf0.1 mg
    MCA2259
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Mouse anti Ovalbumin antibody, clone 2C6 recognises ovalbumin (OVA). The antibody is suitable for use as a mouse IgE standard in ELISA assays (Hamadaet al. 2003).
    • Intended Use
    • Target Species
      Chicken
    • Product Form
      Purified IgE - liquid
    • Reconstitution
    • Preparation
      Purified IgE prepared from tissue culture supernatant.
    • Preservative Stabilisers
      0.09%Sodium Azide
    • Immunogen
      Ovalbumin.
    • Purity
    • Approx. Protein Concentrations
      IgE concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunized Balb/c mice were fused with cells of the mouse myeloma, P3U1.
    • Storage
      Store at +4oC or at -20oC if preferred. Avoid repeated freezing and thawing as this may denature the antibody.

      Storage in frost free freezers is not recommended.

      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/10001/5000

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Ovalbumin Antibody Formats

    Formats Clone Applications Sizes available
    Ovalbumin Antibody : Purified 2C6 E 0.1 mg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rat anti Mouse IgE Heavy Chain:HRPMCA419P0.5 mgE
      MCA419P

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Source Reference

              References

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