Monocytes/Macrophages Antibody | KUL01

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Monocytes/Macrophages Antibody | KUL01 gallery image 1

Published customer image:
Mouse anti Chicken monocytes/macrophages antibody, clone KUL01 used for flow cytometry studies.
Image caption:
Expression of fluorescent protein reporter in MPs requires integration of a full length CSF1R-reporter construct containing chicken FIRE. (A) Southern blot analysis of G1 progeny from G0 founder MAA2-16. DNA isolated from the blood of four G1 birds (lanes 1-4) were digested with restriction enzymes XhoI and ClaI isolate the full-length insert and probed with sequence mApple coding sequence. Expected size of full length insert (5.5 kb) was observed in G1 progeny MAA2-16:13 and MAA2-16:22 (lane 1 and 3), whereas a partially-deleted insert was found in G1 progeny MAA2-16:20 and MAA2-16:33. (B) PCR analysis of G2 progeny (lanes 1-12) from G1 cockerel MAA1-8:23 for the presence of full length (2.5 kb) or partially-deleted (0.5 kb) inserts using primers P1 and P2. (C) Flow cytometric analysis of reporter transgene expression in KUL01+/CSF1R+ blood monocytes in a non-transgenic chicken, G1 cockerel MAA1-8:23 and representative G2 progeny from (B) that have either a full length or a partially-deleted insert.

From: Balic A, Garcia-Morales C, Vervelde L, Gilhooley H, Sherman A, Garceau V, Gutowska MW, Burt DW, Kaiser P, Hume DA, Sang HM. Visualisation of chicken macrophages using transgenic reporter genes: insights into the development of the avian macrophage lineage. Development. 2014 Aug;141(16):3255-65.

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Monocytes/Macrophages Antibody | KUL01 gallery image 2

Published investigator image:
Mouse anti Chicken monocytes/macrophages antibody, clone KUL01 used for flow cytometry studies.
Image caption:
Immunohistochemical evidence of infiltration by T-cells (CD3+), B-cells (chB6+) and macrophages (Kul01+). Immunophenotyping identified the majority of infiltrating lymphocytes as being CD3-positive T-cells (A). They show a characteristic multifocal distribution pattern. With similar spatial characteristics, chB6-positive B-cells comprise the second largest fraction of infiltrating cells (B). Compared to the lymphocytes, the density of Kul01-positive macrophages appears much lower (C). The arrows point to immunopositive cells. All sections were prepared from the same region of the sciatic nerve. The orientation of the nerve fibres (NF) is indicated. Scale bar = 35 µm.

From: Bader SR, Kothlow S, Trapp S, Schwarz SC, Philipp HC, Weigend S, Sharifi AR, Preisinger R, Schmahl W, Kaspers B, Matiasek K. Acute paretic syndrome in juvenile White Leghorn chickens resembles late stages of acute inflammatory demyelinating polyneuropathies in humans. J Neuroinflammation. 2010 Jan 28;7:7.

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  • Mouse anti Chicken Monocytes/Macrophages
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    KUL01
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA5770C, F, P *datasheet pdfdatasheet pdf0.25 mg
    MCA5770
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Chicken Monocytes/Macrophages, clone KUL01 recognises chicken monocytes and macrophages. Clone KUL01 will also detect interdigitating cells and activated microglia but does not recognise Bu1+ve B-cells or CD3 +ve T-lymphocytes.
    • Intended Use
    • Target Species
      Chicken
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by ion exchange chromatography from tissue culture supernatant
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Borate buffered saline.
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      Immunohistology - Frozen
      Immunohistology - Paraffin(1)
      (1)
      This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Monocytes/Macrophages Antibody Formats

    Formats Clone Applications Sizes available
    Monocytes/Macrophages Antibody : Purified KUL01 C, F, P * 0.25 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      STAR117F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      STAR9B
      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      STAR117P
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      STAR12A

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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