IL-2 Antibody | 4F12

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  • Product Type
    Monoclonal Antibody
  • Clone
    4F12
  • Isotype
    IgG2a
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1943ZE, FNdatasheet pdfdatasheet pdf0.25 mg
    MCA1943Z
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Chicken Interleukin-2 antibody, clone 4F12 recognizes natural and recombinant chicken IL-2 expressed in either E.coli or CHO cells.

      Mouse anti Chicken Interleukin-2 antibody, clone 4F12 can be used as the neutralising antibody for bioassays with recombinant chicken IL-2).
    • Intended Use
    • Target Species
      Chicken
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Bovineno
      Humanno
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      None present
    • Immunogen
      Recombinant chicken IL-2
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised mice were fused with cells of the mouse Sp2/0-Ag myeloma cell line.
    • Storage
      Store at -20oC only.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA510 ug/ml
      Functional Assays220 ug/ml

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional IL-2 Antibody Formats

    Formats Clone Applications Sizes available
    IL-2 Antibody : Preservative Free 4F12 E, FN 0.25 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                ELISpot

              References

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