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Horseradish peroxidase conjugated Goat anti Chicken IgM antibody used for the evaluation of flagellin specific IgM response in exposed mice by ELISA.
Kinetics of NHC-specific antibody levels following in ovo vaccination.
ELISA results demonstrating the NHC flagellin-specific reactivity of serially diluted sera collected from (A, C, E) 12 day-old and (B, D, F) 18 day-old chickens injected with 40 μg of NHC flagellin or solvent (control). Serum IgY (A, B), IgM (C, D) and IgA (E, F) antibody responses are indicated. Graphs represent the analysis of individual animals, displayed as a box-and-whisker plot. The amount of antigen-specific antibodies was expressed in a relation to the reference sera (S/R, sample to reference ratio). An asterisk indicates statistically significant differences (α<0.05) between groups.
From: Radomska KA, Vaezirad MM, Verstappen KM, Wösten MMSM, Wagenaar JA, van Putten JPM (2016)
Chicken Immune Response after In Ovo Immunization with Chimeric TLR5 Activating Flagellin of Campylobacter jejuni.
PLoS ONE 11(10): e0164837.