IgA Antibody

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IgA Antibody gallery image 1

Published customer image:
Horseradish peroxidase conjugated Goat anti Chicken IgA antibody (AAI28P) used for the measurement of IgA levels by ELISA.
Image caption:
Antibody levels in APEC- and mock-infected birds following primary infection and after homologous re-challenge. The levels were measured by ELISA using different antigens; IgY levels against soluble χ7122nalR antigens (A), IgY levels against O78 LPS purified from χ7122nalR (B) and IgA levels measured against soluble χ7122nalR antigens (C). At 0, 7, 14, 21, and 28 days post-primary infection, five birds per group were sampled and ten birds per group were sampled after re-challenge (day 42). The data in panels B and C reflect levels at post-mortem examination on day 42, one day after re-challenge. *?=?P?≤?0.05; **?=?P?≤?0.01.

From: Sadeyen JR, Kaiser P, Stevens MP, Dziva F. Analysis of immune responses induced by avian pathogenic Escherichia coli infection in turkeys and their association with resistance to homologous re-challenge.
Vet Res. 2014 45:19.

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IgA Antibody gallery image 2

Published customer image:
Alkalinephosphatase conjugated Goat anti Chicken IgA antibody used for the evaluation of IgA levels in chickens by ELISA
Image caption:
Antigen-specific serum IgM (A), IgA (B), and IgG (C) against S. Virchow and S. Typhimurium at 5, 11, and 26 DPI, following infection of 7-day old chickens. Data shown are based on five birds per group at each time point. Error bars represent the standard error of the mean. An asterisk indicates that there was a significant difference between the S. Virchow infected group and uninfected group (*) or the S. Typhimurium infected group and the uninfected group (**) (P < 0.05).

From: Salisbury A-M, Leeming G, Nikolaou G, Kipar A and Wigley P (2014)
Salmonella Virchow infection of the chicken elicits cellular and humoral systemic and mucosal responses, but limited protection to homologous or heterologous re-challenge.
Front. Vet. Sci. 1:6.

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IgA Antibody gallery image 3

Published customer image:
Horseradish peroxidase conjugated Goat anti Chicken IgA antibody used for the evaluation of flagellin specific IgA response in exposed mice by ELISA.
Image caption:
Antigen-specific serum antibody responses.
NHC flagellin-specific antibody levels in the groups immunized with (A) 40 μg of NHC protein and (B) 20 μg of NHC protein, both compared to levels in the solvent injected control group. Antigen-specific IgY, IgM or IgA antibody levels were determined in 20-fold diluted sera collected from 12 day-old individual animals. Results are displayed as a box-and-whisker plot (S/R, sample to reference ratio). An asterisk indicates statistically significant differences (α<0.05) between compared groups.

From: Radomska KA, Vaezirad MM, Verstappen KM, Wösten MMSM, Wagenaar JA, van Putten JPM (2016)
Chicken Immune Response after In Ovo Immunization with Chimeric TLR5 Activating Flagellin of Campylobacter jejuni.
PLoS ONE 11(10): e0164837.

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IgA Antibody gallery image 4

Published customer image:
Horseradish peroxidase conjugated Goat anti Chicken IgM antibody used for the evaluation of flagellin specific IgM response in exposed mice by ELISA.
Image caption:
Kinetics of NHC-specific antibody levels following in ovo vaccination.
ELISA results demonstrating the NHC flagellin-specific reactivity of serially diluted sera collected from (A, C, E) 12 day-old and (B, D, F) 18 day-old chickens injected with 40 μg of NHC flagellin or solvent (control). Serum IgY (A, B), IgM (C, D) and IgA (E, F) antibody responses are indicated. Graphs represent the analysis of individual animals, displayed as a box-and-whisker plot. The amount of antigen-specific antibodies was expressed in a relation to the reference sera (S/R, sample to reference ratio). An asterisk indicates statistically significant differences (α<0.05) between groups.

From: Radomska KA, Vaezirad MM, Verstappen KM, Wösten MMSM, Wagenaar JA, van Putten JPM (2016)
Chicken Immune Response after In Ovo Immunization with Chimeric TLR5 Activating Flagellin of Campylobacter jejuni.
PLoS ONE 11(10): e0164837.

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IgA Antibody gallery image 5

Published customer image:
Horseradish peroxidase conjugated Goat anti Chicken IgM antibody used for the evaluation of flagellin specific IgM response in exposed mice by ELISA.
Image caption:
Cecal colonization of C. jejuni and sIgA responses in vaccinated and control chickens.(A) Chickens were challenged with C. jejuni at 18 day of age. One week post-inoculation (25 days of age) the number C. jejuni in the ceca was determined by plate counting (CFU/ g). Each symbol represents one chicken of the indicated groups, bars represent the mean number of log10 CFU/ g per group. (B) Antigen-specific sIgA in 20-times diluted cecal content from 25 day-old chickens (one week after C. jejuni challenge) as determined by ELISA. Graphs represent the analysis of individual animals of each of the indicated groups, displayed as a box-and-whisker plot.

From: Radomska KA, Vaezirad MM, Verstappen KM, Wösten MMSM, Wagenaar JA, van Putten JPM (2016)
Chicken Immune Response after In Ovo Immunization with Chimeric TLR5 Activating Flagellin of Campylobacter jejuni.
PLoS ONE 11(10): e0164837.

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  • IgA Antibody thumbnail image 1
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  • Goat anti Chicken IgA:HRP
  • Goat anti Chicken IgA:FITC
  • Goat anti Chicken IgA
  • Goat anti Chicken IgA:Alk. Phos.
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
4 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AAI28ABEdatasheet pdfdatasheet pdf0.5 mg
    AAI28AB
    AAI28PEdatasheet pdfdatasheet pdf1 mg
    AAI28P
    AAI28FC, Fdatasheet pdfdatasheet pdf1 mg
    AAI28F
    AAI28E, IDdatasheet pdfdatasheet pdf1 mg
    AAI28
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Goat anti Chicken IgA antibody specifically recognizes chicken immunoglobulin A and shows no cross-reactivity with other chicken immunoglobulin classes in immunoelectrophoresis.

      Goat anti Chicken IgA antibody may react with IgA from other species.
    • Intended Use
    • Target Species
      Chicken
    • Product Form
      Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
      Purified IgG fraction conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG - liquid
      Purified IgG conjugated to Alkaline Phosphatase - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to chicken IgA were raised by repeated immunisation of goat with highly purified antigen. Purified IgG prepared by affinity chromatography.
    • Preservative Stabilisers
      0.1% Proclin™ 300
      0.2% Bovine Serum Albumin
      0.09%Sodium Azide
      0.2%Bovine Serum Albumin
      0.1% Sodium Azide (NaN3)
      0.09% Sodium Azide (NaN3)
      0.2% Bovine Serum Albumin
    • Immunogen
      Purified chicken IgA.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 0.5mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      50mM HEPES, 0.1M NaCl, 1mM MgCl2, 0.1mM ZnCl2
    • Storage
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC.
      DO NOT FREEZE.

      This product should be stored undiluted.
      Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch.
      18 months from date of despatch.
      12 months from date of despatch.
      12 months from date of despatch.
    • Acknowledgements
      Proclin™ 300 is a trademark of The Dow Chemical Company (“Dow”) or an affiliated company of Dow.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/10,0001/100,000
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      Immunohistology - Frozen1/2001/2,000
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/1001/10000
      Immunodiffusion
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/10001/10000

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional IgA Antibody Formats

    Formats Applications Sizes available
    IgA Antibody : Purified E, ID 1 mg
    IgA Antibody : FITC C, F 1 mg
    IgA Antibody : Alk. Phos. E 0.5 mg
    IgA Antibody : HRP E 1 mg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Goat IgG (Fc):FITCSTAR122F1 mgF
      STAR122F
      Rabbit anti Goat IgG (Fc):HRPSTAR122P1 mgC, E, WB
      STAR122P

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          AbGUARD® HRP Stabilizer PlusBUF052A100 mlC, E, P, WB
          BUF052A
          TMB Signal+BUF054A100 mlE
          BUF054A
          TMB CoreBUF056A100 mlE
          BUF056A
          TMB Core PrestainedBUF057A100 mlE
          BUF057A
          TMB Core+BUF062A100 mlE
          BUF062A
          AbGUARD® HRP Stabilizer PlusBUF052C1000 mlC, E, P, WB
          BUF052C
          AbGUARD® HRP Stabilizer PlusBUF052B500 mlC, E, P, WB
          BUF052B

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                ELISA

              References

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