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CD41/CD61 antibody | 11C3

Mouse anti Chicken CD41/CD61

Product Type
Monoclonal Antibody
Clone
11C3
Isotype
IgG1
Specificity
CD41/CD61

Product Code Applications Pack Size List Price Your Price Qty
MCA2240GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F IF IP 0.1 mg loader
List Price Your Price
loader

Mouse anti Chicken CD41/61, clone 11C3 recognizes chicken integrin CD41/61, also known as GPIIb-IIIa. CD41/61 is a heterodimeric cell surface protein, expressed on chicken thrombocytes and cells of the thrombocyte lineage. Avian thrombocytes are nucleated equivalents of mammalian platelets playing roles in coagulation and phagocytosis.

Mouse anti Chicken CD41/61 clone 11C3 is reported to induce thrombocyte activation (Lacoste-Eleaume et al. 1994).

Target Species
Chicken
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
Carrier Free
Yes
Immunogen
Peripheral blood leukocytes from white leghorn chicken
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the X63/A63-653 myeloma cell line
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This antibody is licensed from INRA-INSERM

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/10 1/50
Immunofluorescence
Immunoprecipitation
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl

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Source Reference

  1. Lacoste-Eleaume, A.S. et al. (1994) Biochemical and functional characterization of an avian homolog of the integrin GPIIb-IIIa present on chicken thrombocytes.
    Exp Cell Res. 213 (1): 198-209.

References for CD41/CD61 antibody

  1. Horiuchi, H. et al. (2004) A monoclonal antibody against chicken thrombocytes reacts with the cells of thrombocyte lineage.
    J Vet Med Sci. 66 (3): 243-50.
  2. Corbel, C. (2002) Expression of alphaVbeta3 integrin in the chick embryo aortic endothelium.
    Int J Dev Biol. 46 (6): 827-30.
  3. Del Cacho, E. et al. (2008) Isolation of chicken follicular dendritic cells.
    J Immunol Methods. 334: 59-69.
  4. Del Cacho, E. et al. (2009) Avian follicular and interdigitating dendritic cells: isolation and morphologic, phenotypic, and functional analyses.
    Vet Immunol Immunopathol. 129: 66-75.
  5. Ody, C. et al. (1999) Glycoprotein IIb-IIIa is expressed on avian multilineage hematopoietic progenitor cells.
    Blood 93: 2898-906.
  6. Chaussé, A.M. et al. (2011) Expression of Toll-like receptor 4 and downstream effectors in selected cecal cell subpopulations of chicks resistant or susceptible to Salmonella carrier state.
    Infect Immun. 79: 3445-54.
  7. Balic, A. et al. (2014) Visualisation of chicken macrophages using transgenic reporter genes: insights into the development of the avian macrophage lineage.
    Development 141: 3255-65.
  8. Meijerink, N. et al. (2020) Early Life Inoculation With Adult-Derived Microbiota Accelerates Maturation of Intestinal Microbiota and Enhances NK Cell Activation in Broiler Chickens.
    Front Vet Sci. 7: 584561.
  9. View The Latest Product References
  10. Meijerink, N. et al. (2021) Analysis of chicken intestinal natural killer cells, a major IEL subset during embryonic and early life.
    Dev Comp Immunol. 114: 103857.
  11. Meijerink, N. et al. (2021) Glucose Oligosaccharide and Long-Chain Glucomannan Feed Additives Induce Enhanced Activation of Intraepithelial NK Cells and Relative Abundance of Commensal Lactic Acid Bacteria in Broiler Chickens.
    Vet Sci. 8 (6): 110.

Synonyms
GPIIb-IIIa

MCA2240GA

152979

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