Beta 2 Microglobulin Antibody | F21-21

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Beta 2 Microglobulin Antibody | F21-21 gallery image 1

Published investigator image:
Mouse anti Chicken Beta 2 Microglobulin antibody, clone F21-21 used for the evaluation of β 2 microglobulin expression on lymphocytes cultured in the presence of peptides overnight by flow cytometry.
Image caption:
Cell binding and assembly analysis of VP2 peptides predicted to bind B12 and B15 class I molecules. Left hand panel, mean fluorescence intensities measuring the increase of F21-21 binding to cells after culture overnight with 1 mM peptide compared to culture overnight without added peptide. Dotted lines indicate the mean fluorescence intensities of the cells without incubation with peptides. Samples were analysed in triplicate, with standard errors well below 10 %. Right hand panel, ratio of the area of peaks corresponding to assembled monomer compared to β2m from size exclusion chromatography of renaturation assays with indicated peptides

From: Butter C, Staines K, van Hateren A, Davison TF, Kaufman J.
The peptide motif of the single dominantly expressed class I molecule of the chicken MHC can explain the response to a molecular defined vaccine of infectious bursal disease virus (IBDV). Immunogenetics. 2013 Aug;65(8):609-18.

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  • Beta 2 Microglobulin Antibody | F21-21 thumbnail image 1
  • Mouse anti Chicken Beta 2 Microglobulin
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  • Product Type
    Monoclonal Antibody
  • Clone
    F21-21
  • Isotype
    IgG1
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA5774C, F, IP, WBdatasheet pdfdatasheet pdf0.25 mg
    MCA5774
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Chicken β2 microglobulin antibody, clone F21-21 recognises chicken β2 microglobulin, a component of MHC class I molecules and is expressed on nearly all nucleated cells.
    • Intended Use
    • Target Species
      Chicken
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Turkeyyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by ion exchange chromatography from tissue culture supernatant
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Borate buffered saline.
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      extracellular region
      immune response
      antigen processing and presentation of peptide antigen via MHC class I
      MHC class I protein complex
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      Immunohistology - Frozen
      Immunoprecipitation
      Western Blotting

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Beta 2 Microglobulin Antibody Formats

    Formats Clone Applications Sizes available
    Beta 2 Microglobulin Antibody : Purified F21-21 C, F, IP, WB 0.25 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)STAR117F0.5 mgF
      STAR117F
      Rabbit F(ab')2 anti Mouse IgG:FITCSTAR9B1 mgF
      STAR9B
      Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)STAR117P0.5 mgE, WB
      STAR117P
      Rabbit F(ab')2 anti Mouse IgG:RPESTAR12A1 mlF
      STAR12A

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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