CFDA-SE

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Resolution of eight cell generations using CFDA-SE

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  • CFDA-SE Cell Proliferation Assay Kit
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  • Product Type
    Accessory Reagent
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    1351201Fdatasheet pdfdatasheet pdf500 µg
    1351201
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
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    References
    Reviews
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    • The CFDA-SE (5[6]-carboxyfluorescein diacetate succinimidyl ester) cell proliferation assay is packaged in 100 μg vials. Simply reconstitute it with DMSO for use and avoid weighing and wasting reagents.

      CFDA-SE is a cell-permeable reagent that is useful in measuring and tracking cell divisions. Upon entering a live cell, the acetate groups of CFDA-SE are cleaved by intracellular esterase to create the fluorescent carboxyfluorescent carboxyfluorescein succinimidyl ester (CFSE) compound. CFSE reacts with free primary amines to create a stable, covalent bond and is retained in the cytosol of cells. As a cell divides, the fluorescence intensity of CFSE is successively halved with each division, allowing each cell generation to be distinguished.
    • Intended Use
    • Product Form
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
      Kit includes 5 x 100 μg vials
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
    • Storage
      Store at -20oC only

      This product is photosensitive and should be protected from light
    • Shelf Life
      Please see label for expiry date.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/401/400

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use
      Important: Thaw all components prior to use.

      Note: The following protocol is a guideline and it should be modified for each experiment as needed.

      1. Prepare a 200 μM stock solution by adding 892.5 μl of DMSO to a CFDA-SE vial and mix by vortexing.

      2. Create a working solution (5-0.5 μM) by diluting the CFDA-SE stock solution from step 1 with your buffer of choice at pH 7.

      3. Resuspend 1 x 106 cells of interest in 500 μl of the working solution.

      4. Incubate the cells for 5-20 min at room temperature. Protect from light.

      5. Centrifuge the sample and remove the supernatant.

      6. Wash the pellet with 3 ml of your buffer of choice.

      7. Resuspend the cells in 500 μl of fresh, prewarmed culture media.

      8. Remove 200 μl to analyze for time zero.

      9. Place the remaining cells in the appropriate conditions for cell proliferation.

      10. Harvest the cells and stain them for the other markers if desired.

      11. Analyze or sort the cells using a flow cytometer or S3™ cell sorter with a 488 nm laser.

    Additional CFDA-SE Formats

    Formats Applications Sizes available
    CFDA-SE : Reagent F 500 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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